ISSN: 0973-7510

E-ISSN: 2581-690X

Research Article | Open Access
Mohd Taufiq Mat Jalil1 , Nurul Aili Zakaria1 and Darah Ibrahim2
1School of Biology, Faculty of Applied Sciences, Universiti Teknologi MARA, 40450, Shah Alam, Selangor, Malaysia.
2Industrial Biotechnology Research Laboratory, School of Biological Sciences, Universiti Sains Malaysia, 11800 Minden, Penang, Malaysia.
J Pure Appl Microbiol. 2021;15(3):1398-1408 | Article Number: 7083
https://doi.org/10.22207/JPAM.15.3.32 | © The Author(s). 2021
Received: 04/06/2021 | Accepted: 02/07/2021 | Published: 31/07/2021
Abstract

The effect of incorporating plant extract in the culture medium on anti-MRSA activity of Lasiodiplodia pseudotheobromae IBRL OS-64, was investigated in the present study. On disk diffusion assay, the ethyl acetate fungal extract from culture medium supplemented with host plant extract (HPE) of Ocimum sanctum leaves demonstrated good anti-MRSA activity with a diameter inhibition zone of 22.6±0.6 mm. Meanwhile, the minimal inhibition concentration (MIC) values of the extract from YES broth and YES broth incorporated with HPE were 1000 µg/mL and 250 µg/mL, respectively. The MBC values were 8000 µg/mL and 500 µg/mL, respectively. The YES + HPE extracts exerted bactericidal effect against the test bacteria since the MBC/MIC ratio was less than or equal to 4. The time-kill study revealed a 90% of growth reduction of MRSA ATCC 33591 after 16 h exposure to the fungal extract cultured in YES + HPE. Ironically, for fungal extract grown in YES broth, time-kill curve showed a regrowth pattern of bacterial cells after 24 h exposed to the extract. Therefore, the present study suggested that the addition of HPE in the culture medium could enhance the anti-MRSA activity of endophytic fungus, L. pseudotheobromae IBRL OS-64 against MRSA ATCC 33591.

Keywords

Anti-MRSA activity, Lasiodiplodia pseudotheobromae, Ocimum sanctum, Minimal Inhibition Concentration (MIC), Minimal Bactericidal Concentration (MBC), Time-kill study

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