Plastics can be considered as the world’s largest pollution that is accumulating in the environment. The degradation of these plastics is difficult because they are accumulated over years and also they are dumped in an area which causes more environmental problems. To reduce the environmental pollution under the waste management with the help of microorganism was taken into account. In those bacteria plays a major role in controlling those problems. Polyurethane can be considered as one of the most used synthetic polymers in the world. Hence, degradation of polyurethane was taken into account in the study with the help of bacteria. In this experiment, we isolated and identified the bacteria that can degrade the polyurethane from the polyurethane buried soil sample. The isolated organism was sequenced and identified at the species level. Enzyme activities of those organisms were also identified along with the degradation process. With the help of this study, we identified a novel bacterium that can degrade polyurethane.