ISSN: 0973-7510

E-ISSN: 2581-690X

Research Article | Open Access
S. Abirami1*, Emilin Renitta R2, Antony V. Samrot3 , M. Sakthikavitha1, P. Revathi1, A. Mini Varsini1, Dhiva S4, S. Saigeetha5, N. Shobana5 and P. Prakash5
1Department of Microbiology, Kamaraj College, Thoothukudi – 628 003, Tamil Nadu, India.
2Department of Food Processing Technology, School of Agriculture and Biosciences, Karunya Institute of Science and Technology, Karunya Nagar, Coimbatore – 641 114, India.
3School of Biomedical Sciences, Faculty of Medicine, Nursing, and Bioscience, MAHSA University, Jalan SP 2, Bandar Saujana Putra, 42610 Jenjarom Selangor, Malaysia.
4Department of Microbiology, Sree Narayana College, Alathur, Palakkad, Kerala – 678 682, Kerala, India.
5Department of Biotechnology, School of Bio and Chemical Engineering, Sathyabama Institute of Science and Technology, Chennai – 600 119, Tamil Nadu, India.
J Pure Appl Microbiol. 2021;15(2):968-975 | Article Number: 6620 | © The Author(s). 2021
Received: 30/08/2020 | Accepted: 25/05/2021 | Published: 01/06/2021

In this study, shells of sea crab and fungus Aspergillus niger were subjected for chitosan extraction which has been done following demineralization, deproteination and deacetylation. Chitosan yield from crab shell and fungi was 37.5% and 39.3% respectively and water binding capacity was 58.44% and 60.21% respectively. The extracted chitosan was characterized using Fourier transform infrared spectroscopy (FTIR) and subjected for antibacterial activity against Urinary tract infection (UTI) pathogens – Klebsiella pneumoniae, Proteus mirabilis and E. coli. Chitosan of crab shell showed better antibacterial activity than fungal derived chitosan. Chitosan gel was prepared using the extracted chitosan where it was also showing good antibacterial activity.


Crab shell, fungal cell, chitosan, antibacterial activity, UTI pathogens

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