ISSN: 0973-7510

E-ISSN: 2581-690X

Research Article | Open Access
Shradhdha Sharma and Duggirala Srinivas Murty
Department of Biogas Research and Microbiology, Gujarat Vidyapith, Sadra – 382 320, Gandhinagar, Gujarat, India.
J Pure Appl Microbiol. 2021;15(2):958-967 | Article Number: 6745 | © The Author(s). 2021
Received: 31/10/2020 | Accepted: 24/05/2021 | Published: 01/06/2021

Nowadays, a lot of interest has been given to the development of cost-effective and efficient enzyme production technologies. Laccase enzymes are widely used in biotechnological, environmental and industrial sectors. Due to the cost-effectiveness of the solid-state fermentation (SSF) process, it is widely used to produce a broad range of biological products. In this study, optimization of moisture content, temperature, pH, and inoculum size were studied to enhance laccase production ability of Pleurotus sajor-caju in SSF by using One Factor At Time (OFAT) and Response Surface Methodology (RSM). OFAT was used as a baseline study for deducing the experimental design of RSM. The highest production of laccase enzyme (1450 U/g) by Pleurotus sajor-caju on wheat straw was observed at 26°C, 6.0 pH, 72.5 % moisture content, 7.5% inoculum size, 1% fructose and 0.5 % peptone. Unlike the conventional inoculum preparation method, here the inoculum was generated by the spawning method for SSF. The molecular weight of partially purified laccase from Pleurotus sajor-caju was estimated to be around 62 K Da using SDS PAGE. The activity staining of laccase was observed as a zymogram on Native PAGE using ABTS as a substrate. Lignin degradation of wheat straw and its structural disruption due to laccase was observed by Scanning Electron Microscopy (SEM).


Ligninolytic enzymes, Response Surface Method (RSM), Scanning Electron Microscopy (SEM)

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