ISSN: 0973-7510

E-ISSN: 2581-690X

Research Article | Open Access
Fatima Moeen Abbas
Department of Biology, College of Sciences for Women, Babylon University, Iraq.
J Pure Appl Microbiol. 2021;15(2):877-882 | Article Number: 6976
https://doi.org/10.22207/JPAM.15.2.43 | © The Author(s). 2021
Received: 08/04/2021 | Accepted: 12/05/2021 | Published: 01/06/2021
Abstract

This study was designed to explore the incidence of blaOXA-1 amongst Klebsiella pneumoniae isolates with resistant to carbapenem. Between December 2014 and April 2015, one hundred samples were taken from two hospitals: Babylon Teaching Hospital for Maternity and Pediatric / Babylon Province (clinical, umbilical infections, n= 40; environmental, n=20) and Karbala Hospital for Pediatric / Karbala Province (40 stool samples). All patients were hospitalized or attended these hospitals, all under 1 year of age. Seventeenth (17%) isolates were identified as Klebsiella pneumoniae. The antibiotic resistance profile of isolates was tested using disk diffusion method. High-level of resistance was recorded with ampicillin (94.1%)  and piperacillin (88.2%)  antibiotics. Resistance to carbapenem was reported in two K.pneumoniae isolates, these were investigated for the existence of OXA-1β-lactamase using Polymerase Chain Reaction (PCR) technique. Two (100%) isolates gave positive result. Transference of this gene was studied by conjugation experiment. The blaOXA-1 gene conjugated successfully in 1 (50%) isolate only.

Keywords

Klebsiella pneumoniae, Carbapenem resistance, OXA-1 β-lactamase, PCR, Conjugation

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