ISSN: 0973-7510

E-ISSN: 2581-690X

Research Article | Open Access
Anish Kumar Sharma , Jyotsana Pandit and Khyati Harkhani
School of Sciences, PP Savani University, Kosamba, Surat, Gujarat-394125, India.
J Pure Appl Microbiol. 2021;15(2):777-786 | Article Number: 6559
https://doi.org/10.22207/JPAM.15.2.31 | © The Author(s). 2021
Received: 31/07/2020 | Accepted: 17/05/2021 | Published: 26/05/2021
Abstract

A total of seventy-two bacterial isolates were obtained employing enrichment culture technique from apple orchard soils contaminated with chlorpyrifos. Pure cultures of bacterial isolates were obtained using streak plate method on mineral salt medium. Bacterial isolates were characterized on the basis of morphology, culture and biochemical properties. Six bacterial isolates exhibited high extracellular organophosphorus hydrolase activity along with high tolerance towards high concentrations of chlorpyrifos. Genomic DNA extraction from bacterial isolates was done with phenol/chloroform method. Molecular Diversity of six chlorpyrifos degrading bacterial isolates was done employing RAPD-PCR technique by using 25 decamer primers, where amplification was showed by only 20 primers. A total of 337 amplified bands and 64 unique bands ranging in size from 100 to 4900bps were produced after RAPD analysis. The similarity coefficient estimated by Jaccard’s coefficient for these bacterial isolates was found to range between 31 to 64 percent.

Keywords

Chlorpyrifos, Enrichment Technique, Molecular Diversity, Organophosphorus hydrolase (OPH), Random amplification of polymorphic DNA (RAPD)

Article Metrics

Article View: 33

Share This Article

© The Author(s) 2021. Open Access. This article is distributed under the terms of the Creative Commons Attribution 4.0 International License which permits unrestricted use, sharing, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.