ISSN: 0973-7510

E-ISSN: 2581-690X

Research Article | Open Access
Tahani F. H. Alahmadi1,2,3, Ziab Z. Alahmadey3, Sameer R. Organji1,2,Khaled Elbanna1,2,4, Iqbal Ahmad5 and Hussein H. Abulreesh1,2
1Department of Biology, Faculty of Applied Science, Umm Al-Qura University, Makkah, Saudi Arabia.
2Research Laboratories Unit, Faculty of Applied Science, Umm Al-Qura University, Makkah, Saudi Arabia.
3Ohud Hospital, Ministry of Health, Madinah, Saudi Arabia.
4Department of Agricultural Microbiology, Faculty of Agriculture, Fayoum University, Fayoum, Egypt.
5Department of Agricultural Microbiology, Faculty of Agricultural Sciences, Aligarh Muslim University, Aligarh 202002, India.
J Pure Appl Microbiol. 2021;15(2):725-734 | Article Number: 6973
https://doi.org/10.22207/JPAM.15.2.24 | © The Author(s). 2021
Received: 04/04/2021 | Accepted: 06/05/2021 | Published: 26/05/2021
Abstract

We report in this study for the first time the prevalence of multiple resistant Staphylococcus haemolyticus in clinical settings in Saudi Arabia. A total of 1060 clinical specimens of hospitalized patients were screened for the presence of S. haemolyticus in the period between September and December 2020. Primary identification of the isolates was carried out by colonial characteristics on mannitol salt agar and clumping factor test, confirmation of presumptive isolates and antimicrobial susceptibility testing was performed by Vitek® 2, while PCR was employed to detect mecA and vanA genes. A total of 20 S. haemolyticus isolates were recovered from 20 samples (blood cultures, urine, nasal swab, wound swab, groin swab, and axilla swab), 90% (P <0.001, x2) of the isolates were multiple resistant to three antimicrobial agents and more. Resistance to oxacillin was exhibited in 95% of the isolates, while none of the isolates were resistant to vancomycin and linezolid, yet resistance to rifampicin was observed in 30 % of the isolates. The findings of this study highlights the emerging trends of Staphylococcus haemolyticus as potential drug resistant pathogen in hospital settings in Saudi Arabia, which requires in depth investigation on molecular understanding on antimicrobial resistance and virulence traits of the strains.

Keywords

Staphylococcus haemolyticus, nosocomial, antimicrobial resistance, mecA, vanA, Saudi Arabia

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© The Author(s) 2021. Open Access. This article is distributed under the terms of the Creative Commons Attribution 4.0 International License which permits unrestricted use, sharing, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.