Staphylococcus aureus (S.aureus)  is a prevalent organism causing infections in the community and hospital. A variety of antibiotics are used, including the Macrolide-Lincosamide-StreptograminB (MLS_B) family of antibiotics in which clindamycin is the preferred agent. Widespread use of these antibiotics leads to resistance to these MLS_B antibiotics; a D-test can characterize the different MLS_B phenotypes. This study was taken up with an objective to perform a double disc diffusion test for detecting different phenotypes in S.aureus with particular reference to inducible clindamycin resistance. Out of a total of 174(100%) strains of S.aureus, 98(56.32%) were MRSA, and 76(43.68%) were MSSA. All isolates were tested by D-test. A total of 47(27.01%) were of cMLS_B phenotype, 31(17.82%) were of iMLS_B phenotype, and 96(55.17%) were of MS phenotype. The majority of MRSA strains were cMLS_B phenotype(76.60%) and iMLS_B phenotype (64.52%) in comparison to MSSA isolates. Although iMLS_B phenotypes are present in both MRSA and MSSA, iMLSB was more in MRSA isolates. Appropriate susceptibility data is essential for a clinician to start clindamycin therapy to prevent therapeutic failures with inducible MLS_B resistance in S.aureus isolates. It will be appropriate for all the clinical laboratories to report inducible Clindamycin resistance in S.aureus strains (both MRSA and MSSA), for which D-test is a reliable testing method.