ISSN: 0973-7510

E-ISSN: 2581-690X

Research Article | Open Access
Giridhar Kumar Modukuru, Pradeep Madala Sobhana Surya,
Vishnuvardhana Rao Kakumanu and Saritha Yarava
Department of Microbiology, Dr. Pinnamaneni Siddhartha Institute of Medical Sciences and Research Foundation, Chinnavutapalli, Gannavaram Mandal, Krishna -521 286, Andhra Pradesh, India.
J Pure Appl Microbiol. 2021;15(2):689-694 | Article Number: 6768 | © The Author(s). 2021
Received: 13/11/2020 | Accepted: 09/03/2021 | Published: 12/05/2021

Staphylococcus aureus (S.aureus)  is a prevalent organism causing infections in the community and hospital. A variety of antibiotics are used, including the Macrolide-Lincosamide-StreptograminB (MLSB) family of antibiotics in which clindamycin is the preferred agent. Widespread use of these antibiotics leads to resistance to these MLSB antibiotics; a D-test can characterize the different MLSB phenotypes. This study was taken up with an objective to perform a double disc diffusion test for detecting different phenotypes in S.aureus with particular reference to inducible clindamycin resistance. Out of a total of 174(100%) strains of S.aureus, 98(56.32%) were MRSA, and 76(43.68%) were MSSA. All isolates were tested by D-test. A total of 47(27.01%) were of cMLSB phenotype, 31(17.82%) were of iMLSB phenotype, and 96(55.17%) were of MS phenotype. The majority of MRSA strains were cMLSB phenotype(76.60%) and iMLSB phenotype (64.52%) in comparison to MSSA isolates. Although iMLSB phenotypes are present in both MRSA and MSSA, iMLSB was more in MRSA isolates. Appropriate susceptibility data is essential for a clinician to start clindamycin therapy to prevent therapeutic failures with inducible MLSB resistance in S.aureus isolates. It will be appropriate for all the clinical laboratories to report inducible Clindamycin resistance in S.aureus strains (both MRSA and MSSA), for which D-test is a reliable testing method.


Staphylococcus aureus, MRSA, MSSA, D-test, inducible clindamycin resistance, MLSB phenotypes

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© The Author(s) 2021. Open Access. This article is distributed under the terms of the Creative Commons Attribution 4.0 International License which permits unrestricted use, sharing, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.