ISSN: 0973-7510

E-ISSN: 2581-690X

Research Article | Open Access
Saud Bawazeer1, Abdur Rauf2 , Humaira Naz3, Syed Uzair Ali Shah4, Dania Saad Waggas5, Jawad Ali2, Yahia N. Mabkhot6, Faryal7 and Mohamed Fawzy Ramadan8
1Department of Pharmaceutical Chemistry, Faculty of Pharmacy, Umm Al-Qura University, Makkah, P.O. Box 42, Saudi Arabia.
2Department of Chemistry, University of Swabi, Swabi, Anbar, KP, Pakistan.
3Department of Zoology, Shaheed Benazir Bhutto Women University Peshawar, KP, Pakistan.
4Department of Pharmacy, University of Swabi, Swabi, KP, Pakistan.
5Pharmacology, Fakeeh College for Medical Sciences, Jeddah, Saudia Arabia.
6Department of Pharmaceutical Chemistry, College of Pharmacy, King Khalid University, Abha 61421, Saudi Arabia.
7Institute of Chemical Sciences, University of Peshawar, KP, Pakistan.
8Biochemistry Department, Faculty of Agriculture, Zagazig University, Zagazig 44519, Zagazig, Egypt.
J Pure Appl Microbiol. 2021;15(2):667-676 | Article Number: 6819 | © The Author(s). 2021
Received: 18/12/2020 | Accepted: 30/03/2021 | Published: 01/05/2021

Trachyspermum ammi seeds were selected for photochemical study. The crude T. ammi methanol and aqueous extracts showed the presence of alkaloids, saponins, steroids, terpenoids, coumarins, betacyanins, flavonoids and soluble starch. The synthesis of gold nanoparticles (AuNPs) using T. ammi extract was characterized using UV-visible, and FT-IR spectroscopy. The appearance of sharp peak at 520 nm in the UV visible spectra, and the appearance of broad band nanoparticles spectra at 563.21 cm-1, 516.92 cm-1 and 462.92 cm-1 as well as the disappearance of the carboxyl OH bond and carbon-carbon triple bond supported the formation of AuNPs. The crude T. ammi methanol and AuNPs were investigated for antioxidant potential using DPPH· free radical assay, which shows that crude extract has significant antioxidant effect. The synthesized AuNPs was also evaluated for antibacterial activities against staphylococcus aureus, Klebsiella pneumonia and Bacillus subtilis. The crude extract showed activity against Bacillus subtilis, while AuNPs showed activity against staphylococcus aureus. The in vivo sedative effect and analgesic effect were enhanced in AuNPs treated animals in 5 times less dose (i.e., 5, and 10 mg/kg) than that of crude extract. It was concluded that T. ammi extract includes capping and reducing agents, which make it capable to be developed as stable AuNPs. The biological action of AuNPs is either enhanced (sedative and analgesic) or changed (antibacterial activity), when compared with that of plant extract.


Nanotechnology, phytochemicals, biological activities, sedative effect, analgesic effect

Article Metrics

Article View: 846

Share This Article

© The Author(s) 2021. Open Access. This article is distributed under the terms of the Creative Commons Attribution 4.0 International License which permits unrestricted use, sharing, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.