ISSN: 0973-7510

E-ISSN: 2581-690X

Research Article | Open Access
Mohamad Adib Syahmi Latif1, Fazilah Ariffin1,2 , Rudiyanto3, Shahrul Ismail4, Noorazrimi Umor5, Abu Zahrim Yaser6 and Zaharah Ibrahim7
1Faculty of Science and Marine Environment, Universiti Malaysia Terengganu, 21030 Kuala Nerus, Terengganu, Malaysia.
2Biological Security and Sustainability Research Group, Universiti Malaysia Terengganu, 21030 Kuala Nerus, Terengganu, Malaysia.
3Faculty of Fisheries and Food Science, Universiti Malaysia Terengganu, 21030 Kuala Nerus, Terengganu, Malaysia.
4Faculty of Ocean Engineering Technology and Informatics, Universiti Malaysia Terengganu, 21030 Kuala Nerus, Terengganu, Malaysia.
5Department of Microbiology, Faculty of Applied Science, Universiti Teknologi MARA Negeri Sembilan Branch, Kuala Pilah Campus, 72000 Kuala Pilah, Negeri Sembilan, Malaysia.
6Faculty of Engineering, Universiti Malaysia Sabah, Jalan UMS, 88400 Kota Kinabalu, Sabah, Malaysia.
7Faculty of Science, Universiti Teknologi Malaysia, 81310, UTM Johor Bahru, Johor, Malaysia.
J Pure Appl Microbiol. 2021;15(2):658-666 | Article Number: 6859 | © The Author(s). 2021
Received: 17/01/2021 | Accepted: 24/03/2021 | Published: 01/05/2021

Biological treatment for textile wastewater always has a limitation in term of time of reaction and uncertainty along the process. This study focused on the decolorization of synthetic azo dyes in batch reactors with controlled thermotolerant anaerobic conditions. Less-volatile digested sludge collected from a palm oil biogas reactor was used as the organic biodegradation agent for azo dyes. Digested sludge contains high amounts of microbes with uncertain species viable for decolorization purposes. Sodium acetate trihydrate (C2H9NaO5) was used as carbon source and mixed with a specific composition of minimum salt media (MSM) in batch reactors as an additional nutrient. Digested sludge both in mesophilic (35°C) and thermophilic (55°C) conditions were found to be capable of decolorizing 100, 200 and 300 ppm of three types of azo dyes: Reactive Green 19 (45.56%, 69.73%; 63%, 73.49%; 70.02%, 75.92%), Reactive Orange 16 (46.08%, 78.4%; 64.21%, 85.52%; 74.95%, 85.91%) and Reactive Red 120 (29.11%, 85.32%; 63.35%, 87.69%; 72.02%, 89.5%) respectively after 7 days incubation time. Statistical analysis also showed that the anaerobic thermophilic conditions had significantly accelerated the decolorization process. The anaerobic thermophilic environment will be a good factor to include in future textile wastewater treatment plants.


Anaerobic, Continuous Stirred Tank Reactor, Digested Sludge, Azo Dye, Thermotolerant

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© The Author(s) 2021. Open Access. This article is distributed under the terms of the Creative Commons Attribution 4.0 International License which permits unrestricted use, sharing, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.