ISSN: 0973-7510

E-ISSN: 2581-690X

Research Article | Open Access
G.H. Sewify1, H.M. Hamada2, Z.A. Mustafa3 and Hani A. Alhadrami4,5
1Deanship of Scientific Research, King Abdulaziz University, P.O. Box 80230 Jeddah 21589, Saudi Arabia.
2Department of Economic Entomology and Pesticides, Faculty of Agriculture, Cairo University, Giza, Egypt.
3Faculty of Dentistry, Department of Oral Diagnostic Science King Abdulaziz University, P.O. Box 80230 Jeddah 21589, Saudi Arabia.
4Faculty of Applied Medical Sciences, Department of Medical Laboratory Technology, King Abdulaziz University, P.O. Box 80402, Jeddah 21589, Saudi Arabia.
5Molecular Diagnostics Lab, King Abdulaziz University Hospital, King Abdulaziz University, P.O. Box 80402, Jeddah 21589, Saudi Arabia.
J Pure Appl Microbiol. 2021;15(2):613-620 | Article Number: 6769
https://doi.org/10.22207/JPAM.15.2.03 | © The Author(s). 2021
Received: 13/11/2020 | Accepted: 12/03/2021 | Published: 31/03/2021
Abstract

In vitro study was conducted to explore antibacterial properties of the larval gut extracts of Rhynchophorus ferrugineus (Red Palm Weevil) Oliver. Larval gut extracts were tested against salivary bacteria causing dental carries using the agar well diffusion method. The gut extracts significantly affected the growth of both Klebsiella spp. and Streptococcus viridans. The two bacterial species revealed significant differences in their sensitivity to the extract. The extract efficacy depended upon the concentration and time of exposure. When using 100%concentration of the extract, the mean of inhibition zones for S. viridans and Klebsiella spp. at 24 h after treatment were 1.61 mm and 2.50 mm, respectively. At 48 h post-treatment, the mean of inhibition zones for S. viridans and Klebsiella spp. were 1.96 mm and 2.66 mm. After 72 hours, the means zones were 2.28 mm and 2.91 mm, respectively. Electron microscopic examinations showed morphological changes of the outer membrane of bacteria with a noticeable damage as a result of exposure to the gut extract. The results suggest potential use of these extracts against dental caries bacteria.

Keywords

Rhynchophorus ferrugineus, Red Palm Weevil, Antimicrobial activity, Streptococcus viridians, Klebsiella spp.

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