ISSN: 0973-7510

E-ISSN: 2581-690X

Research Article | Open Access

Hormoz Selahvarzi1, Milad Kamdideh1, Mehrnoosh Vahabi1, Ali Dezhgir1, Massoud Houshmand2,3 and Soha Sadeghi4,5

1Department of Biology, Tehran North branch, Islamic Azad University, Tehran, Iran.
2Research Center, Knowledge University, Erbil, Kurdistan Region, Iraq.
3Department of Medical Genetics, National Institute of Genetic Engineering and Biotechnology (NIGEB), Tehran, Iran.
4Human Biology and Medical Genetics, Sapienza University, 00185 Rome, Italy.
5Department of Genetics, Research institute of Royesh Gene, Karaj, Iran.
J Pure Appl Microbiol. 2021;15(1):201-211 | Article Number: 6804 | © The Author(s). 2021
Received: 07/12/2020 | Accepted: 01/02/2021 | Published: 09/02/2021

This study was carried out to investigate the relationship between common variants in two vitamin D pathway genes (VDR and CYP27B1) and vitamin D3 serum levels. In this study, serum vitamin D metabolite levels were measured in the blood samples of 200 patients with alopecia areata. Then, single nucleotide polymorphisms (SNPs) in VDR and CYP27B1 were analyzed using polymerase chain reaction (PCR)-sequencing. Sixty-three variations were observed in these genes (42 variations in CYP27B1 and 21 variations in VDR). A significant difference in Rs1544410 (odds ratio: 7, P < 0.0005) and rs4646536 (odds ratio: 4.043, P < 0.0005) variants was found between the patients and controls. The study showed the relationship between the two polymorphisms, Rs1544410 (odds ratio: 7, 95% CI, 1–8) and rs4646536 (odds ratio: 4.043, 95% CI, 3–14.038) on the genes VDR and CYP27B1, respectively, with increased risk of developing vitamin D3 insufficiency in the Iranian population. Therefore, SNPs in the VDR and CYP27B1 genes can be considered as prognostic biomarkers of the risk of developing vitamin D3 deficiency.


VDR, CYP27B1, VITAMIN D3, RS 1544410, SNPs

Article Metrics

Article View: 42

Share This Article

© The Author(s) 2021. Open Access. This article is distributed under the terms of the Creative Commons Attribution 4.0 International License which permits unrestricted use, sharing, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.