ISSN: 0973-7510

E-ISSN: 2581-690X

Research Article | Open Access
Abigail R. Sopia1,2, Pushpa Innocent D. Joseph3, M. Kalyani2, B. Ananthi1, Suresh Dhanaraj4 and Allen John Henry4
1A.C.S. Medical College and Hospitals, Chennai – 600077, Tamil Nadu, India.
2Saveetha Institute of Medical and Technical Sciences, Chennai – 600077, Tamil Nadu, India.
3Karpaga Vinayaga Medical Sciences and Research Center, Kanchipuram – 603308, Tamil Nadu India.
4Vels Institute of Science Technology and Advanced Studies, Chennai – 600117, Tamil Nadu, India.
J Pure Appl Microbiol. 2021;15(1):194-200 | Article Number: 6645
https://doi.org/10.22207/JPAM.15.1.14 | © The Author(s). 2021
Received: 10/09/2020 | Accepted: 12/01/2021 | Published: 09/02/2021
Abstract

Genital infections and subsequent vaginosis diagnosed through high vaginal swab in women is caused due to fungi or bacteria. The presented study focused on determining the types, numbers and antibacterial susceptibility pattern of aerobic bacteria causing vaginosis in 147 female patients attending infertility centre in Chennai, Tamil Nadu, India. Candida spp. caused 17% of infections with 15.7% of vaginosis caused by E.coli, Klebsiella spp., Acinetobacter spp., Citrobacter spp. and Gram positive cocci – Methicillin Sensitive Staphylococcus aureus (MSSA), Coagulase Negative Staphylococci(CONS), Staphylococcus aureus and Enterococcus spp. causing 12.9% of vaginitis in the study population. A total of 20 different antibiotics – cell wall inhibitors, protein synthesis inhibitors and nucleic acid synthesis inhibitors; were tested to determine the response of bacterial isolates by Kirby-Bauer disc diffusion method. The study result determined that the most effective drug for treating Gram positive bacterial vaginitis as per CLSI guidelines based on susceptibility pattern as: Linezolid(100%), Gentamycin(91.6%), Amikacin(87.5%),Erythromycin(79.2%), Co-Trimoxazole(72.2%), Ciprofloxacin(65.6%) and least Chloramphenicol(44.3%). High level gentamycin(83.3%) was found to be effective in treating Enterococci. The descending order of susceptibility of Gram negative aerobacteria causing vaginitis as per CLSI guidelines are: Amikacin(87.5%), Gentamycin(82.5%), Cefoperazone sulbactam (76.3%), Ciprofloxacin(68.5%), Ceftazidime(62.5%) and least Amoxyclav(25%).All Gram negative bacteria tested were susceptible to– Imipenem and Meropenem as well as Chloramphenicol. Ceftriaxone (87.5%) and Nitrofurantoin(72.3%) among other antibiotics was effective against Gram negative bacteria while all Enterobacteriaceae members were found to be resistant to tetracycline.

Keywords

Vaginitis, Antibiotic Susceptibility pattern, Imipenem, Meropenem, Amikacin, CLSI guidelines, Nitrofurantoin

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© The Author(s) 2021. Open Access. This article is distributed under the terms of the Creative Commons Attribution 4.0 International License which permits unrestricted use, sharing, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.