ISSN: 0973-7510

E-ISSN: 2581-690X

Research Article | Open Access
Rasha M. El-Mekkawy1, Taghreed N. Almanaa2, Marwa A. Yassin1,Gamal Rabie1 and Noha Saleh1
1Botany and Microbiology Department, Faculty of Science, Zagazig University, Zagazig, Egypt.
2Department of Botany and Microbiology, College of Science, King Saud University, Riyadh, Saudi Arabia.
J Pure Appl Microbiol. 2021;15(1):105-113 | Article Number: 6644 | © The Author(s). 2021
Received: 10/09/2020 | Accepted: 25/12/2020 | Published: 23/01/2021

The antimicrobial agents of silver nanoparticles (AgNPs) have been applied a little while back in diverse therapeutic studies. In this analysis, AgNPs were biosynthesized using an ecologically welcomed and cost-effective simple of bio-reduction. An isolate of Aspergillus flavus KF946095 (A. flavus) was found to biosynthesize AgNPs; the size of AgNPs was (56nm) and detected by UV-Vis analysis at (400 nm). The reducing properties for biosynthesis of AgNPs are mainly due to the protein functional surface reactive groups detected by Fourier Transform Infrared spectroscopy (FTIR).Whereas, FTIR for AgNPs showed different peaks at 3994.5, 3201.6, 1801.4, 1643.2 and 1604.7 cm-1 that shared with the biosynthesize and stability of AgNPs as protein capping agents. Transmission Electron Microscope (TEM) confirmed the scattering of biosynthesized AgNPs within a sol with oval and round shapes. The antibiotic susceptibility test was studied for some pathogenic bacteria. Staphylococcus aureus DSM 1104 (S. aureus) appeared to be the more resistant strain; it resisted the action of 6 antibiotics out of 8 ones tested. MIC value of AgNPs was 20µg/mL and antibiotic ciprofloxacin was 30µg/mL. Mixture of MIC values or double MIC values distinctively inhibited the multidrug resistant (MDR) S.aureus.


Silver nanoparticles, Aspergillus flavus, FTIR, Staphylococcus aureus, antibacterial activity

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© The Author(s) 2021. Open Access. This article is distributed under the terms of the Creative Commons Attribution 4.0 International License which permits unrestricted use, sharing, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.