ISSN: 0973-7510

E-ISSN: 2581-690X

Review Article | Open Access

Ragini Bhatia1, Rajesh Chaudhary2, Sandip Kumar Khurana3, Ruchi Tiwari4, Kuldeep Dhama5 , Vivek Kumar Gupta6, Raj Kumar Singh7 and Senthilkumar Natesan1

1Indian Institute of Public Health Gandhinagar, Lekawada, Ganghinagar, Gujarat – 382042, India.
2General Surgeon, Civil Hospital, Nagrota Bagwan, Himachal Pradesh – 176047, India.
3ICAR-Central Institute for Research on Buffaloes, Sirsa Road, Hisar, 125001, India.
4Department of Veterinary Microbiology and Immunology, College of Veterinary Sciences, UP Pandit Deen Dayal Upadyaya Pashu Chikitsa Vigyan Vishwavidyalya Evam Go-Anusandhan Sansthan (DUVASU), Mathura 281001, Uttar Pradesh, India.
5Division of Pathology, ICAR-Indian Veterinary Research Institute, Izatnagar, Bareilly 243122, India.
6Centre for Animal Disease Research and Diagnosis, ICAR-Indian Veterinary Research Institute, Izatnagar, Bareilly 243122, India.
7ICAR-Indian Veterinary Research Institute, Izatnagar, Bareilly – 243 122, India.
J Pure Appl Microbiol. 2020;14(suppl 1):789-798 | Article Number: 6354
Received: 06/05/2020 | Accepted: 10/05/2020 | Published: 14/05/2020

Severe acute respiratory syndrome corona virus-2 (SARS-CoV-2), a novel coronavirus initially reported in Wuhan, China, is the causative agent of coronavirus disease (COVID-19) pandemic. Symptoms of the disease comprise of fever, tiredness, dry cough, aches and pains, nasal congestion, runny nose, sore throat, diarrhoea and pneumonia at the late stage. SARS-CoV-2 has severely crippled the healthcare system and has caused huge economic losses. Following the outbreak, the SARS-CoV-2 was recognized timely and its genome was sequenced, leading to the development of real-time polymerase chain reaction assays for its detection in clinical samples collected from suspected cases. The management of the pandemic is limited by a number of misconceptions and insufficient information about laboratory testing for SARS-CoV-2 to confirm the disease. This includes a lack of awareness about procedures for the collection, transport, testing, and handling of biological samples for COVID diagnosis. This article provides an overview of the current laboratory diagnostic methods with a purpose to provide information and guidance to laboratories, stakeholders, broader community and especially public health professionals involved in laboratory testing for SARS-CoV-2.


COVID-19, SARS-CoV-2, molecular diagnosis, RT-PCR, prevention, control

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