ISSN: 0973-7510

E-ISSN: 2581-690X

Research Article | Open Access
Hala Mohammed Majeed1 , Siham Agmee Wadee2 and Waseem Ali Hasan2
1Department of Basic sciences , College of Medicine/ Ibn Sina University of Medicine and Pharmaceutical Sciences, Baghdad, Iraq.
2Department of Pharmacology and Biochemisty, Collage of Veterinary Medicine, University of Tikrit, Iraq.
J Pure Appl Microbiol. 2020;14(4):2637-2648 | Article Number: 6090
https://doi.org/10.22207/JPAM.14.4.40 | © The Author(s). 2020
Received: 11/02/2020 | Accepted: 11/03/2020 | Published: 09/11/2020
Abstract

The bacteria have been noted as the main cause of late wound healing. The greatest common pathogen causing the wound contaminations is Staphylococcus aureus. The current study was carried out to isolate and diagnose the staphylococcus aureus which causes of open wound inflammation after surgery in mice process and to study the effect of nickle nanoparticles solution on bacterial isolated and evaluating the molecular and pathological techniques. The study included the collection of 60 cotton swabs from the Office of the Consultant of the Faculty of Veterinary Medicine – Tikrit University and from the external veterinary clinics (from November 2018 to March 2019) from the areas of contaminated wounds or inflamed after surgery. The results of the laboratory cultural of 60 cotton swabs used showed to isolated 50(83.3%) Staphylococcus aureus isolated. And all bacterial isolates were resistant to Doxycycline hydrochloride, Penicillin, CO-Trimoxazole, Ciprofloxacin, Cephalosporin and Penicillin. The study showed that the NFNPS used to inhibit the growth of bacterial isolated by using different concentrations the MBC killer concentration was 256μg / L and the lowest inhibitory concentration to Staphylococcus aureus was MIC 64 microgram / L). Molecular studies included the observation of the most important molecular changes at the level of DNA prior to and treatment with nanoparticles. Many variations were observed on the studied bacterial isolated Including the appearance and disappearance of DNA and its different numbers when treated with nanoparticles.As for the results of the histopathological, it was found that the injury of mice with Staphylococcus aureus antibiotic resistance emerged after about five days and the symptoms were heat, redness and swelling of the skin and the release of yellow and green purulent secretions from the place of injury. When treated mice infected with nanoparticles and antibiotics together the time of the healing was faster than the time of the healing of nanoparticles treated only.

Keywords

Effect, Nickle, nanoparticles, staphylococcus aureus, Contaminated wounds, healing, mice

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