ISSN: 0973-7510

E-ISSN: 2581-690X

Research Article | Open Access
H.A. Akinyele1 , A.A.T. Taliat2, G.C. Enwerem2, O.G. Dawodu2 and O.S. Owojuyigbe2
1Federal University Oye-Ekiti, Ekiti State, Nigeria.
2Federal Polytechnic, Ede, Osun State, Nigeria.
J Pure Appl Microbiol. 2020;14(4):2585-2593 | Article Number: 6128
https://doi.org/10.22207/JPAM.14.4.34 | © The Author(s). 2020
Received: 01/03/2020 | Accepted: 07/07/2020 | Published: 26/11/2020
Abstract

Fungi are plentiful in nature and they are found growing on wastes of wood materials. These wastes are equally found in our environment with no usefulness. The aim of this study was to exploit the probability of the isolated fungi from fruits to produce cellulase from wastes of lignocellulosic materials. Cellulase- producing fungi were isolated from fruits (tomato, banana, plantain). The organisms were screened for cellulase production. Culture conditions were optimized with pH, temperature and carbon. Cellulase was produced using lignocellulosic wastes; sawdust, corn cob, sugarcane bagasse. Six cellulase producers were isolated, four of which were selected for synthesis and quantification of the cellulase. The fungi were identified as Saccharomyces cerevisiae4, Trichoderma species1 and Scopulariopsis brevicaulis1. Of all the tested substrates used in this study, pretreated sugarcane bagasse at 3% w/v concentration with Scopulariopsis brevicaulis gave highest cellulase production 18.18 U/mL at 40°C, 5day incubation time and pH 5, followed by Trichoderma with 12.39 U/mL. These fungi are good potentials cellulase producers that can be considered at industrial level.

Keywords

Fungi, peptone, substrate, plantain, cellulase

Article Metrics

Article View: 183

Share This Article

© The Author(s) 2020. Open Access. This article is distributed under the terms of the Creative Commons Attribution 4.0 International License which permits unrestricted use, sharing, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.