ISSN: 0973-7510

E-ISSN: 2581-690X

Research Article | Open Access
Sami Bawazeer1, Abdur Rauf2, Khaliq Ur Rahman2, Jawad Ali2, Ghias Uddin3, Fatima Begum3, Mohammad S. Mubarak4 and Mohamed Fawzy Ramadan5,6
1Pharmacognosy Department, College of Pharmacy, Umm Al-Qura University, Makkah, Saudia Arabia.
2Department of Chemistry, University of Swabi, Anbar-23561, Khyber Pakhtunkhwa, Pakistan.
3Institute of Chemical Sciences, University of Peshawar, K.P.K Peshawar – 25120, Pakistan.
4Department of Chemistry, The University of Jordan, Amman, 11942, Jordan.
5Deanship of Scientific Research, Umm Al-Qura University, Makkah, P.O.Box 715, Saudi Arabia.
6Agricultural Biochemistry Department, Faculty of Agriculture, Zagazig University, Zagazig – 44519, Egypt.
J Pure Appl Microbiol. 2020;14(4):2513-2523 | Article Number: 6682 | © The Author(s). 2020
Received: 27/09/2020 | Accepted: 03/11/2020 | Published: 09/11/2020

The aim of the current study was to detect various secondary metabolites in the extract of Debregeasia salicifolia, and to synthesize stable silver/gold nanoparticles (Ag/AuNPs) from D. salicifolia methanol crude extract. The antioxidant, antibacterial, and antifungal activities of the methanol crude extract, various isolated fractions, and the synthesized nanoparticles were evaluated. Phytochemical analyses of the methanol extract/fractions indicated the presence of tannins, saponins, flavonoids, steroids, terpenoids, coumarins, emodins, and soluble starch. Gold and silver nanoparticles have been subsequently synthesized from the methanol crude extract by green synthesis, and characterized by UV and IR spectroscopic techniques. Size of the particles was determined with the aid of an atomic force microscope (AFM). The results revealed that the size of AuNPs was 5-100 nm, and that of AgNPs was in the range 5-100 nm. Bioactivity screening revealed that chloroform and n-hexane fractions exhibited significant 2,2-diphenyl-1-picrylhydrazyl (DPPH·) radical scavenging potential against quercetin. Additionally, the extract/fractions and AuNPs were also subjected to antimicrobial activity screening against a number of strains of microbes. Among extracts, n-hexane fraction showed good antifungal activity as compared to other fractions, whereas in the case of anti-bacterial activity, extract and fractions were active against Gram positive and Gram negative bacterial strain. AuNPs were exhibited moderate activity against all tested bacterial strains.


Green synthesis, Atomic force microscope, antiradical, antioxidant, antimicrobial

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© The Author(s) 2020. Open Access. This article is distributed under the terms of the Creative Commons Attribution 4.0 International License which permits unrestricted use, sharing, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.