ISSN: 0973-7510

E-ISSN: 2581-690X

Research Article | Open Access
Aya A. Khodier1, Amal Saafan2, Walid Bakeer3 and Ahmed S. Khairalla3,4,5
1Department of Microbiology and Immunology, Faculty of Pharmacy Fayoum University, Fayoum, Egypt.
2Department of Microbiology and Immunology, Faculty of Pharmacy Menoufia University, Egypt.
3Department of Microbiology and Immunology, Faculty of Pharmacy Beni-Suef University, Egypt.
4Department of Biology, University of Regina, Regina, Saskatchewan, Canada.
5Department of Biology – University Credit – Coast Mountain College, British Columbia, Canada.
J Pure Appl Microbiol. 2020;14(4):2399-2405 | Article Number: 6638
https://doi.org/10.22207/JPAM.14.4.17 | © The Author(s). 2020
Received: 09/09/2020 | Accepted: 28/10/2020 | Published: 30/12/2020
Abstract

Acinetobacter baumannii is an opportunistic microorganism commonly found in intensive care units (ICUs), and it is responsible for a broad span of hospital-acquired infections. Persistence of nosocomial infection caused by multidrug-resistant (MDR) A. baumannii is an alarming health care issue in Egypt, and at present, colistin remains the treatment of choice for the management of MDR A. baumannii infections. A. baumannii possesses great capacity to develop and acquire resistance to a broad range of antibiotics. The acquisition and dissemination of antibiotic-resistant determinants in A. baumannii strains are mediated by integrons, especially class I integrons. This study focuses on the characterization of some genetic mechanisms underlying the multidrug-resistant phenotypes of A. baumannii isolates in Egypt. Forty-eight A. baumannii specimens were isolated from different hospitalized patients; least resistance was observed against amikacin and tigecycline, with 60% and 58.5% of the isolates resistant, respectively, whereas 62.5% of the isolates were resistant to imipenem and meropenem. The highest sensitivity was found for colistin. Genetic analysis revealed that blaoxa-51 was detected in all isolates, the blaoxa-23-like gene was detected in 80% of the isolates, and blaoxa-24 and blaoxs-58 were not detected in any isolate. Finally, PCR analysis revealed that 6.6% of isolates carried the class I integron gene.

Keywords

Acinetobacter baumannii, Multidrug-resistant (MDR)

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