ISSN: 0973-7510

E-ISSN: 2581-690X

Research Article | Open Access
Anhar Al-Turk1, Nidal Odat2 and Muhannad I. Massadeh1
1The Hashemite University, Faculty of Science, Department of Biology and Biotechnology, 13115, Al-Zarqa, Jordan.
2Al-Balqa Applied University, Faculty of Science, Department Medical Labratories, 19117, Al-Salt, Jordan.
J Pure Appl Microbiol. 2020;14(4):2363-2370 | Article Number: 6639 | © The Author(s). 2020
Received: 09/09/2020 | Accepted: 03/10/2020 | Published: 04/11/2020

Currently, there is an increase prevalence of antibiotic-resistant bacteria worldwide. Therefore, the need for characterization of naturally occuring antibiotics with less antibiotic resistance is required. Soil resources contains valuable antibiotic producing microorganisms that increasingly being utilized for the production of suitable antibiotics. Therefore, this study aimed at identifying an antibiotic bacteria with ability of producing antibiotic that is isolated from soil samples collected from Al Zarqa provenance, an arid area in Jordan. Morphological and biochemical characterization of the isolates were carried out and found that all of the isolates belong to Bacillus genus. Further confirmation of the characterization of the bacteria was done by ribosomal RNA and PCR. The results reveal that the isolates represent Basilluslicheniformis. These bacilli were further investigated for antimicrobial activities against 6 ATCC human pathogens viz., S. aureus, S. pneumonia, Salmonella typhi., E. coli, P. mirabels and E. cloacae. Additionally, the results of Gas Chromatography Mass Spectrometry (GCMS) of ethyl acetate extracts for B. licheniformis secondary metabolites showed that they contain two main antimicrobial compounds namely Pyrrolo [1, 2-a] pyrazine-1, 4-dione,hexahydro and Trans-13-octadecenoic acid. The present work maybe suggests that soil isolates from the studied arid area include antibiotic producing strains that can be utilized commercially.


Bacillus, Antibacterial activity, 16S rRNA, GCMS

Article Metrics

Article View: 195

Share This Article

© The Author(s) 2020. Open Access. This article is distributed under the terms of the Creative Commons Attribution 4.0 International License which permits unrestricted use, sharing, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.