ISSN: 0973-7510

E-ISSN: 2581-690X

Research Article | Open Access
Viviana Pamela Chiluisa-Utreras1 , Katherine Alejandra Medrano Jara2, Andrés Sebastián Cadena Paredes2 and Ramiro Daniel Acurio1
1BIOARN Research Group, Universidad Politécnica Salesiana, Quito, Ecuador.
2Laboratories of Life Sciences, Universidad Politécnica Salesiana, Quito, Ecuador.
J Pure Appl Microbiol. 2020;14(4):2339-2349 | Article Number: 6633
https://doi.org/10.22207/JPAM.14.4.12 | © The Author(s). 2020
Received: 04/09/2020 | Accepted: 15/12/2020 | Published: 25/12/2020
Abstract

Phytopathogenic diseases are a major concern in modern agriculture, and for decades, pesticides have been used to prevent potential damage. Bacillus megaterium is proposed as a biological controlling agent, and gene expression of the lipopeptide genes FEND and ITUDI was assessed using RT-qPCR. Inhibition effects of B. megaterium on Alternaria sp. and Botrytis sp. were examined over a period of nine days, which confirmed the potential use of this bacterium to counteract these two pathogens. In addition, expression of FEND and ITUDI genes was assessed over nine days in the aforementioned dual cultures and inhibition tests. FEND expression in B. megaterium increased 20.16-fold in response to Alternaria sp., and ITUDI expression increased 3.20-fold in response to Botrytis sp. on day five of incubation. These results were corroborated by gene expression data obtained from B. megaterium during fermentation, where FEND and ITUDI gene expression increased 95.14- and 18.70-fold, respectively. In conclusion, B. megaterium can increase lipopeptide synthesis when exposed to these particular phytopathogens and can significantly increase the respective expression during fermentation.

Keywords

Gene expression, Antagonism, Antimicrobial genes, Plant pathogens

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