ISSN: 0973-7510

E-ISSN: 2581-690X

Research Article | Open Access
Anupam Berwal1, K.L. Shobha1 , Rohit Gupta1, Kriti Gupta2,Asem Ali Ashraf1, Abishek Kumar B1, Raghav Chawla1, Prerna Aggarwal3, Bhawna Sharma3 and Sonali4
1Department of Microbiology, Kasturba Medical College, Manipal, Manipal Academy Of Higher Education, Manipal, Karnataka, India.
2Dr. Niraj Kumar Gupta MicroPath Lab, Bijnor (U.P.) India.
3Department of Microbiology, Kalpana Chawla Govt. Medical College, Karnal, India.
4Department of Pathology, Kalpana Chawla Govt. Medical College, Karnal, India.
J Pure Appl Microbiol. 2020;14(3):2033-2038 | Article Number: 6154 | © The Author(s). 2020
Received: 10/03/2020 | Accepted: 07/08/2020 | Published: 19/09/2020

Non fermenting gram-negative bacilli (NFGNB) are recently striving as uropathogens. The present study was conducted to isolate the common species of bacteria in NFGNB causing urinary tract infection (UTI) and its correlation with comorbid conditions and to study the antibacterial susceptibility pattern. This retrospective study was done at the diagnostic Microbiology laboratory of a tertiary care hospital. Urine samples were collected for the period of six months. These samples were plated on blood agar and MacConkey agar and incubated at 37°C for 18–24 hr under aerobic conditions. Identification of NFGNB was done by Gram staining and MALDI-TOF (Matrix- Assisted Laser Desorption/ Ionization- Time of Flight, Biomerieux- Diagnostics). Antibiotic sensitivity testing was done by Vitek® 2 system (Biomerieux- Diagnostics) using N 281 card. Data was analyzed using SPSS IBM version 16. Out of the total 16,413 non repetitive urine samples that were received in the laboratory, 318 had significant bacteriuria. NFGNB were identified in 108 (33.9%) of all the urine samples with significant bacteriuria. Prevalence of non-fermenters in our study was 0.6%. NFGNB were more frequently isolated in the females and also in the age group of more than 50 years. Eighty five (78.70%) had comorbid conditions. P. aeruginosa and A. baumannii were the most common organism isolated among NFGNB. Pseudomonas aeruginosa isolates showed high susceptibility to imipenem (80.2%) and amikacin (66.6%). NFGNB although seen frequently in females and in age group of 50 years and above, clinical correlation with comorbid condition is essential to label it as uropathogens. Amikacin or imipenem may be the empirical drug of choice.


NFGNB, comorbid condition, Antibiotic susceptibility, Bacteriuria, Urinary tract infection

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© The Author(s) 2020. Open Access. This article is distributed under the terms of the Creative Commons Attribution 4.0 International License which permits unrestricted use, sharing, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.