ISSN: 0973-7510

E-ISSN: 2581-690X

Research Article | Open Access
Pooja Pushkaran, Dinta Daniel, Sivaramakrishnan Subramaniyan and
G.S. Sandhia
Department of Botany and Research Centre, University College, University of Kerala, Trivandrum 695 034, Kerala, India.
J Pure Appl Microbiol. 2020;14(3):1949-1960 | Article Number: 6279
https://doi.org/10.22207/JPAM.14.3.34 | © The Author(s). 2020
Received: 24/04/2020 | Accepted: 16/07/2020 | Published: 09/09/2020
Abstract

Plants with medical properties are often enriched with endophytes that have the potential to produce   important   bioactive compounds. Endophytes after entering the plant tissue may either colonize a particular tissue or may  spread throughout the host plant without causing damage. The possession of pharmaceutical and biological properties has made the Holostemma ada-kodien Schult as one of the widely used plants of medicinal importance in India. Following the direct cut method three endophytic bacterial strains (UC H1, UC H4 and UC H7) were isolated, identified and characterized from the healthy looking rhizome of H. ada-kodien. Among these isolates, UC H1 and UC H4 were found to have many properties like antibacterial compounds, hydrolytic enzymes and plant growth promoting traits. The isolate UC H4 have ability for Indole-3-Acetic Acid (IAA) production of 513.54 U/ml and very good protease and pectinase activities of 20.65 U/ml and 16.09 U/ml respectively. So far no reports are available on the endophytic microflora of H. ada-kodien.

Keywords

Endophytic bacteria, 16S rRNA gene sequencing, antibacterial activity, IAA production, RP-HPLC,  phosphate solubilisation, ACC deaminase production

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