Dodonaea viscosa (Family: Sapindaceae) is a medicinal plant that has been used as anti-rheumatic and bone pain in Peru as a folk remedy. Ethanol extract obtained from D. viscosa leaves was partitioned as n-hexane fraction; chloroform fraction; ethyl acetate fraction; n-butanol fraction and aqueous fraction. Phytoconstituents from D. viscosa leaves were evaluated by using chemical reagents to identify the presence of each phytochemical. In addition, a cytotoxic effect was determined by the Sulforhodamine B (SRB) test. The results showed that the ethanol extract and ethyl acetate fraction of leaves had the highest variety of phytoconstituents. The ethanol extrac of leaves exhibited a major inhibitory effect compared to different fractions in human colon cancer cells (HT-29). From these assays, it is concluded that D. viscosa does not possess any detectable cytotoxic effect on epidermal cells from mouse (3T3), and a slight cytotoxic effect against HT-29 tumor cells compared to 5-FU. This species could appear like a good source of herbal medicine in colorectal cancer disease.