ISSN: 0973-7510

E-ISSN: 2581-690X

Research Article | Open Access
Isra Tayseer1 , Hanan Azzam2,3, Nehaya Al-Karablieh2,4, Amal Mayyas5 and Talal Aburjai1
1Department of Pharmaceutical Sciences, School of Pharmacy, The University of Jordan, Amman, Jordan.
2Hamdi Mango Center for Scientific Research, The University of Jordan, Amman, Jordan.
3Department of Chemistry, Faculty of Science, The University of Jordan, Amman, Jordan.
4Department of Plant Protection, School of Agriculture, The University of Jordan, Amman, Jordan.
5Faculty of Health sciences, American University of Madaba, Madaba, Jordan.
J Pure Appl Microbiol. 2020;14(3):1855-1861 | Article Number: 6517 | © The Author(s). 2020
Received: 05/07/2020 | Accepted: 26/08/2020 | Published: 08/09/2020

The present study was conducted to assess the in vitro activities of folk medicinal plants in combination with levofloxacin against TG1 and mutant KAM3-1(∆acrB-∆tolC) Escherichia coli strains. Plants were chosen based on their traditional use in combination with antibiotics among laymen. Standard protocols were followed to examine the antimicrobial activity of plant extracts and levofloxacin against E. coli in term of their minimum inhibitory concentrations (MICs) and to evaluate the plant extracts-levofloxacin interaction using checkerboard method. Among the twelve plants investigated, Thymus vulgaris, Zingiber officinale, Teucrium polium, Matricaria chamomilla and Curcuma longa had the best antimicrobial activities against E. coli strains with MIC values at 250 μg/ml. It is noteworthy to mention that other folk plants extracts reveled no effects against E coli strains. Furthermore, additive interactions were observed between levofloxacin and T. polium or T. vulgaris against E. coli wild-type TG1 strain. There was no antagonism being observed in this study. The detection of additive interaction between the extracts and levofloxacin demonstrates the prospective of these folk medicinal plants as a source of compounds to modulate antibiotic resistance.


Interaction, Escherichia coli, levofloxacin, antibiotics, Minimum inhibition concentration, checkerboard

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