ISSN: 0973-7510

E-ISSN: 2581-690X

Research Article | Open Access

Abdelkader Benhelima1,3 , Olivier Vidal2, Zohra Kaid-Omar3, Rabea Sahki4 and Jean-Marie Lacroix2

1Department of Process Engineering, Faculty of Technology, Dr. Tahar Moulay University, Saida 20000, Algeria.
2Structural and Functional Glycobiology Unit (UGSF) CNRS UMR 8576, University of Sciences and Technologies Lille, Cedex 59655, France.
3Department of Medicine, Faculty of Medicine, Djillali Liabes University, Sidi-Bel-Abbes 22000, Algeria.
4National Forest Research Institute (INRF), Tamanrasset 11000, Algeria.
J Pure Appl Microbiol. 2020;14(3):1835-1844 | Article Number: 6257
https://doi.org/10.22207/JPAM.14.3.22 | © The Author(s). 2020
Received: 08/04/2020 | Accepted: 05/08/2020 | Published: 24/08/2020
Abstract

Three plants adopted by nomads at Tassili n’ajjer (south Algeria) in traditional medicine namely, Cymbopogon schoenanthus, Anabasis articulata and Salvia chudaei, were analysed for theirs antibacterial, antibiofilm and antioxidant properties. Total flavonoid and phenolic contents were measured with 2% AlCl3 and Folin-Ciocalteu’s reagent method, respectively. The antibacterial propertie was investigated by measurement of MIC of plants extract inhibing bacterial proliferation. The antibiofilm propertie was calculated by fluorescent quantization of the DAPI labeled bacterial biomass fixed on the surface and by COMSTAT analysis of confocal scanning laser microscopy (CSLM) images. DPPH radical scavenging and β-carotene/linoleate bleaching methods were used to determine the antioxidant activities of the plants. Total phenolic content was ranged from 21.98 to 2.51 (gallic acid equivalents mg/g methanolic extracts) and the total flavonoid content ranged from 19.27 to 1.65 (catechin equivalent mg/g methanolic extracts). Antibacterial activity against four Gram positive bacteria was shown with plants extracts. The biofilm inhibition concentration of extracts decreasing 50% of biofilm cell density (BIC50) for Salvia chudaei and Cymbopogon schoenanthus extracts ranges from 1 to 10 µg/mL. CSLM images analysis revealed that both surface covering by germ and three dimensional development of the biofilm were reduced with plants extracts. For antioxidant activities, the methanol extracts of the plants evaluated showed low antioxidant activity, with a IC50 between 1.94 and 6.16 mg/ml. Thus, Our systematic research showed that this three common plants of Sahara desert has diversified phytochemicals possessing satisfying extent of antimicrobial, antibiofilm and antioxidant activities

Keywords

Saharian plants, methanolic extracts, antibacterial, antibiofilm, antioxidant

Article Metrics

Article View: 309

Share This Article

© The Author(s) 2020. Open Access. This article is distributed under the terms of the Creative Commons Attribution 4.0 International License which permits unrestricted use, sharing, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.