ISSN: 0973-7510

E-ISSN: 2581-690X

Research Article | Open Access
Zulhabri Othman1,3 , Fasehah Ali2 and Asdren Zajmi2
1Postgraduate Centre, Management and Science University, Seksyen 13, 40100 Shah Alam, Selangor Malaysia.
2Faculty of Health and Life Sciences, Management and Science University, Seksyen 13, 40100 Shah Alam, Selangor, Malaysia.
3International Centre for Halal Studies, Management and Science University, Seksyen 13, 40100 Shah Alam, Malaysia.
J Pure Appl Microbiol. 2020;14(3):1809-1816 | Article Number: 6456 | © The Author(s). 2020
Received: 15/01/2020 | Accepted: 12/08/2020 | Published: 17/09/2020

Traditional medicinal plant possessed antimicrobial properties. Candidiasis is an infection of Candida albicans which has developed resistance towards antifungal drugs. The extracts of Murraya koenigii Spreng leaves and Etlingera elatior Jack flowers were used as antifungal agents individually and in combination against C. albicans. Both extracts were tested for the presence of phytochemicals (alkaloids, tannins, flavonoids, glycosides and saponins). Individual extracts were examined for antifungal activity using Kirby-Bauer test. The minimum inhibitory concentration (MIC) and the minimum fungicidal concentration (MFC) were evaluated using microdilution broth assay and checkerboard assay. The extracts of both plants were positive for the presence of phytochemicals flavonoids, alkaloids, tannins, glycosides and saponins. The inhibition zone of M. koenigii leaf and E. elatior flower extracts were 8.33 mm and 8.17 mm respectively. The MIC of M. koenigii and E. elatior ranged from 200 mg/mL to 400 mg/mL and in combination, the extracts were indifferent towards C. albicans (FIC=1.0). MFC revealed there was no visible growth of C. albicans on SDA plate. M. koenigii and E. elatior have potential to be used individually or in combination as antifungal agents against C. albicans.


Antifungal, Candida albicans, Etlingera elatior, Murraya koenigii, synergistic

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© The Author(s) 2020. Open Access. This article is distributed under the terms of the Creative Commons Attribution 4.0 International License which permits unrestricted use, sharing, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.