ISSN: 0973-7510

E-ISSN: 2581-690X

Research Article | Open Access
Muhammad Nisar1, Shujaat Ali Khan2, Maryam Gul2, Abdur Rauf3,
Salman Zafar2 and Mohamed Fawzy Ramadan4,5
1Office of Research, Innovation and Commercialization, University of Peshawar, Peshawar – 25120, Pakistan.
2Institute of Chemical Sciences, University of Peshawar, Peshawar – 25120, Pakistan.
3Department of Chemistry, University of Swabi, Swabi, Anbar – 23561, KPK, Pakistan.
4Deanship of Scientific Research, Umm Al-Qura University, Makkah, P.O.Box 715, Saudi Arabia.
5Biochemistry Department, Faculty of Agriculture, Zagazig University, Zagazig – 44519, Zagazig, Egypt.
J Pure Appl Microbiol. 2020;14(3):1789-1800 | Article Number: 6605
https://doi.org/10.22207/JPAM.14.3.17 | © The Author(s). 2020
Received: 22/08/2020 | Accepted: 16/09/2020 | Published: 24/09/2020
Abstract

The aim of the current research finding was to synthesize, characterize and antibacterial evaluation of sparfloxacin-mediated noble metal nanoparticles. Noble metal [silver (Ag), and gold (Au)] nanoparticles (NPs), mediated with fluoroquinolone, an anti-bacterial drug [Sparfloxacin, (Sp)], was synthesized by a facile and convenient procedure. Formulated Ag-Sp NPs, and Au-Sp NPs exhibited stability against variation in pH, NaCl solution, temperature, and time. The structural topographies of Ag-Sp, and Au-Sp NPs were determined by fourier transform infrared spectroscopy (FTIR), UV-visible spectroscopy (UV-Vis), scanning electron microscopy (SEM) atomic force microscopy (AFM), and energy dispersive X-ray (EDX). UV-Vis revealed the formulation of NPs by showing typical surface Plasmon absorption maxima at 410 nm for Ag-Sp NPs and 555 nm for Au-Sp NPs. The AFM and SEM analysis ascertained stable mono dispersed Ag-Sp NPs and Au-Sp NPs in the size range of 40-50 nm, and 70-80 nm, respectively. Ag-Sp, and Au-Sp NPs exhibited antibacterial traits against Bacillus subtilis, Staphylococcus aureus, and Klebsiella pneumonia, showing a zone of inhibition (ZOI) ranging from 20±0.98 mm to 24±0.94 mm (Ag-Sp NPs), and 22±0.79 mm to 26±0.92 mm (Au-Sp NPs) at dose of 3 mg/mL.

Keywords

Sparfloxacin, Silver, Gold, UV-VIS spectroscopy, FTIR, AFM, SEM, Anti-bacterial activity

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