ISSN: 0973-7510

E-ISSN: 2581-690X

Research Article | Open Access
Muhammad Nisar1, Shujaat Ali Khan2, Maryam Gul2, Abdur Rauf3,
Salman Zafar2 and Mohamed Fawzy Ramadan4,5
1Office of Research, Innovation and Commercialization, University of Peshawar, Peshawar – 25120, Pakistan.
2Institute of Chemical Sciences, University of Peshawar, Peshawar – 25120, Pakistan.
3Department of Chemistry, University of Swabi, Swabi, Anbar – 23561, KPK, Pakistan.
4Deanship of Scientific Research, Umm Al-Qura University, Makkah, P.O.Box 715, Saudi Arabia.
5Biochemistry Department, Faculty of Agriculture, Zagazig University, Zagazig – 44519, Zagazig, Egypt.
J Pure Appl Microbiol. 2020;14(3):1789-1800 | Article Number: 6605 | © The Author(s). 2020
Received: 22/08/2020 | Accepted: 16/09/2020 | Published: 24/09/2020

The aim of the current research finding was to synthesize, characterize and antibacterial evaluation of sparfloxacin-mediated noble metal nanoparticles. Noble metal [silver (Ag), and gold (Au)] nanoparticles (NPs), mediated with fluoroquinolone, an anti-bacterial drug [Sparfloxacin, (Sp)], was synthesized by a facile and convenient procedure. Formulated Ag-Sp NPs, and Au-Sp NPs exhibited stability against variation in pH, NaCl solution, temperature, and time. The structural topographies of Ag-Sp, and Au-Sp NPs were determined by fourier transform infrared spectroscopy (FTIR), UV-visible spectroscopy (UV-Vis), scanning electron microscopy (SEM) atomic force microscopy (AFM), and energy dispersive X-ray (EDX). UV-Vis revealed the formulation of NPs by showing typical surface Plasmon absorption maxima at 410 nm for Ag-Sp NPs and 555 nm for Au-Sp NPs. The AFM and SEM analysis ascertained stable mono dispersed Ag-Sp NPs and Au-Sp NPs in the size range of 40-50 nm, and 70-80 nm, respectively. Ag-Sp, and Au-Sp NPs exhibited antibacterial traits against Bacillus subtilis, Staphylococcus aureus, and Klebsiella pneumonia, showing a zone of inhibition (ZOI) ranging from 20±0.98 mm to 24±0.94 mm (Ag-Sp NPs), and 22±0.79 mm to 26±0.92 mm (Au-Sp NPs) at dose of 3 mg/mL.


Sparfloxacin, Silver, Gold, UV-VIS spectroscopy, FTIR, AFM, SEM, Anti-bacterial activity

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© The Author(s) 2020. Open Access. This article is distributed under the terms of the Creative Commons Attribution 4.0 International License which permits unrestricted use, sharing, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.