Morphological and DNA-based Classification of Cyathus sp. Isolates from Thailand (Basidiomycota, Nidulariaceae)

ISSN: 0973-7510

E-ISSN: 2581-690X

Research Article | Open Access

Morphological and DNA-based Classification of Cyathus sp. Isolates from Thailand (Basidiomycota, Nidulariaceae)

Waraporn Sutthisa¹ and Niwat Sanoamuang^2,3

¹Department of Biology, Faculty of Science, Mahasarakham University, Kantarawichai District, Maha Sarakham Province, 44150 Thailand.

²Department of Plant Science and Agricultural Resources, Faculty of Agriculture, Khon, Kaen University, Khon Kaen, 40002 Thailand.

³Applied Taxonomic Research Center, Khon Kaen University, Khon Kaen, 40002 Thailand.

J Pure Appl Microbiol. 2020;14(3):1769-1777 | Article Number: 6433

https://doi.org/10.22207/JPAM.14.3.15 | © The Author(s). 2020

Received: 25/05/2020 | Accepted: 13/08/2020 | Published: 04/09/2020

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Abstract

Cyathus sp. isolates from three areas in Thailand (Khon Kaen University, Nam Nao National Park and Phu Ruea, Loei Province) were morphologically characterized by their peridiocarp, peridioles and basidiospores. This allowed to assign most isolates to five Cyathus species: C. berkeleyanus, C. earlei, C. pallidus, C. stercoreus and C. striatus. Phylogenetic analysis of ribosomal ITS sequence data yielded three groups of Cyathus isolates and unidentified species group. The Pallidum group including KKUNN1, is closely related to C. berkeleyanus (DQ463355.1), KKUITN2 (KU202745) and KKUITN3 (KU202751) are closely related to C. pallidus (DQ463356.1). The Ollum group includes C. africanus and C. hookeri. The Striatum group, such as KKUITP2 (KU202744) and KKUITP3 (KU202743) are closely related to C. stercoreus (DQ463356.1). The LSU sequence data suggest that KKULN2 and KKULN3 are closely related to C. pallidus (DQ463336.1), whereas KKULP2 and KKULP3 are closely related to C. stercoreus.

Keywords

Cyathus, ribosomal internal transcribed spacer (ITS), large subunit ribosomal DNA (LSU rDNA), peridiocarp

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