ISSN: 0973-7510

E-ISSN: 2581-690X

Research Article | Open Access
Elif Erkaya1, Berna Genc2, Sumeyya Akbulut1, Gulsah Adiguzel3, Mehmet Akif Omeroglu1, Hakan Ozkan1 and Ahmet Adiguzel1
1Department of Molecular Biology and Genetics, Faculty of Science, Ataturk University, 25400, Erzurum, Turkey.
2Department of Genetic and Bioengineering, Faculty of Engineering and Life Sciences, Gumushane University, 29100, Gumushane, Turkey.
3Department of Food Science and Technology, Faculty of Veterinary Science, Ataturk University, 25400, Erzurum, Turkey.
J Pure Appl Microbiol. 2020;14(2):1567-1576 | Article Number: 6416
https://doi.org/10.22207/JPAM.14.2.55 | © The Author(s). 2020
Received: 20/05/2020 | Accepted: 27/06/2020 | Published: 29/06/2020
Abstract

In this study, traditional sausage samples from different provinces of Turkey (Gaziantep, Antalya, Erzurum and Kahramanmaras) were obtained and one hundred three isolates were collected. Using the (GTG)5-PCR genomic fingerprint analysis method, seven of them were observed to be different and conventional tests of these isolates were performed. Molecular identification of two isolates carrying the bacteriocin gene and having antimicrobial activity by agar disc diffusion method was performed by 16S rRNA sequence analysis. As a result, the seven isolates were identified as Aerococcus urinaeequi (EK1), Streptococcus salivarius (EK2), Leuconostoc mesenteroides (EK3), Macrococcus caseolyticus (EK4), Lactococcus garvieae (EK5), Staphylococcus saprophyticus (EK6) and Lactobacillus sakei (EK7). Among these strains, it has been determined that Ln. mesenteroides and L. sakei carried the mecentericin and sacacin genes. When antimicrobial activity against different strains was examined, inhibition formations of Ln. mesenteroides and L. sakei on Enterococcus faecalis, Shigella dysenteriae and Escherichia coli O157: H7 were observed.

Keywords

Bacteriocin, (GTG)5-PCR, 16S rRNA sequence analysis, antimicrobial activity

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