ISSN: 0973-7510

E-ISSN: 2581-690X

Research Article | Open Access
Yogeswari Subramaniam1 , Ramalakshmi Subbiah2, Leeba Balan1 and Kamalraj Subban1
1Centre for Advanced Studies in Botany, University of Madras, Maraimalai Campus, Guindy, Chennai – 600 025, Tamil Nadu, India.
2Department of Microbiology, Sri Moogambigai Arts and Science College for Women, Palacode-Hosur Main Road, Thimmarayanahalli PO, Mallupatti, Dharmapuri Dst.- 636 805, (Tamil Nadu), India.
J Pure Appl Microbiol. 2020;14(2):1557-1566 | Article Number: 6076
https://doi.org/10.22207/JPAM.14.2.54 | © The Author(s). 2020
Received: 07/02/2020 | Accepted: 06/06/2020 | Published: 29/06/2020
Abstract

In the present study, to optimize the media for the production of bioactive compounds from Monochaetia karstenii was carried out and compounds were identified by GC-MS. M. karstenii was identified from infected Camellia japonica leaves by classical and molecular taxonomy. It was cultured in different media and determined their mycelial biomass and antibacterial activity. Further Maltose Maltose tartrate broth (MTB) was altered for its media components such as carbon, nitrogen, minerals, amino acids and vitamins sources and physical parameters like temperature, pH and incubation periods for growth and production of secondary metabolites from M. karstenii. The antimicrobial and antioxidant compounds were performed from three different solvent extracts (Chloroform, Dichloromethane and Ethyl acetate) of M. karstenii from optimized medium. M. karstenii had optimum growth in MTB showing mycelial growth of 13.16 g/L.  The ethyl acetate extract observed significant antibacterial activity against Escherichia coli (21 mm), Staphylococcus aureus (20 mm) and Vibrio chloreae (18 mm). In-vitro antioxidant activity revealed that, the IC50 values for 2, 2’-azino-bis (3-ethylbenzothiazoline-6-sulphonic acid (ABTS), 2, 2-diphenyl-1-picrylhydrazyl (DPPH) and total antioxidant radical scavenging assay of 100.58 μg/ml, 140 μg/ml and 141.91 μg/ml from ethyl acetate extract respectively. Thus the antimicrobial and antioxidant activity of the fungal extract has been due to the presence of biocompounds such as cyclohexenone derivatives, cinnamic acid, isooxazoline 3-phenyl- benzodiazepine, 2- propenoic acid 3-phenyl-(E)-dodecene and 3-undecen -1-yne (E) were characterized by gas chromatography-mass spectrometry (GC–MS) and reported first time in M. karstenii. We conclude that M. karstenii possess excellent antimicrobial and antioxidant potential and can be exploited for the discovery of new drug molecules.

Keywords

Monochaetia karstenii, anti-bacterial, antioxidant, GC-MS analysis, media optimization, cinnamic acid

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© The Author(s) 2020. Open Access. This article is distributed under the terms of the Creative Commons Attribution 4.0 International License which permits unrestricted use, sharing, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.