ISSN: 0973-7510

E-ISSN: 2581-690X

Research Article | Open Access
Khwaja Salahuddin1 , Md. Nizamuddin Ansari2, Chandan Kumar Singh2, Anupama3, Amit Kumar Jha3, Ritisha N. Tatmiya4 and Syeda Fauzia Farheen Zofair5
1Department of Botany, M.R.M. College, L.N. Mithila University, Darbhanga – 846 004, Bihar, India.
2Tissue Culture Lab, University Department of Botany, BRA. Bihar University, Muzaffarpur – 842 001, Bihar, India.
3Department of Zoology, MRM College, L.N. Mithila University, Darbhanga – 846 004, Bihar, India.
4Department of Biology, Christ College, Saurashtra University, Rajkot – 362 002, Gujarat, India. 5Interdisciplinary Biotechnology Unit, Aligarh Muslim University, Aligarh,202002, Uttar Pradesh, India.
J Pure Appl Microbiol. 2020;14(2):1495-1502 | Article Number: 6013
https://doi.org/10.22207/JPAM.14.2.47 | © The Author(s). 2020
Received: 30/12/2019 | Accepted: 05/05/2020 | Published: 24/05/2020
Abstract

This study is aimed at isolating and characterizing strains of an actinomycetes from soils. Six strains of actinomycetes were isolated from the riverside, rhizoplane and low land of Muzaffarpur district of Bihar (India). The cultural characterisation revealed the production of diffusible pigments of different colours. Heavy sporulation was noticed in some strains. Out of six strains isolated, four strains such as KS-1, KS-2, KS-3 and KS-4 were found to be mesophilic, and the other two KS-5 and KS-6 showed thermophilic when subjected to a higher temperature. The antibiotic susceptibilities of these six strains gave the idea about the sensitive and resistance nature based on the zone of inhibition against six antibiotics. Three strains (KS-1, KS-3 and KS-5) were found to be sensitive, whereas KS-2, KS-4 and KS-6 exhibited resistance to the particular antibiotic. The antibiogram of these strains such as KS-2, KS-4 and KS-6 clearly showed the different levels of resistance such as constitutive resistance, lethal dose50 and minimal inhibitory concentration within the susceptible range of different antibiotics.  CR was found to be maximum (3.5 µgmL-1) in strain KS-4 against tetracycline, the highest lethal dose (14µgmL-1) was noticed in KS-2 against ampicillin while Minimal Inhibitory concentration was found to be maximum (26µgmL-1) against streptomycin in strain KS-6.

Keywords

Constitutive resistance, Lethal dose, Minimal inhibitory concentration

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