ISSN: 0973-7510

E-ISSN: 2581-690X

Research Article | Open Access

 C. Pooja, Mita D. Wadekar , S. Jayashree and J.V. Sathish

Department of Microbiology, Chamarajanagar Institute of Medical Sciences, Chamarajanagar – 571 313, Karnataka, India.
J Pure Appl Microbiol. 2020;14(2):1323-1328 | Article Number: 6266
https://doi.org/10.22207/JPAM.14.2.28 | © The Author(s). 2020
Received: 17/04/2020 | Accepted: 22/06/2020 | Published: 27/06/2020
Abstract

Dengue and Chikungunya are mosquito-borne arboviral infections with Aedes aegypti being the common vector for both. Manifestations of dengue fever closely resemble with the manifestations of Chikungunya. Although Chikungunya is a self-limiting disease, but dengue causes serious complications like Dengue Hemorrhagic Fever and Dengue Shock Syndrome. Detection of IgM antibody in Dengue and Chikungunya infection helps in early diagnosis and treatment. To study the Prevalence and Seasonal Trends of Dengue and Chikungunya infection. Two years retrospective study was conducted from January 2017 to December 2018. Serum samples from patients who were clinically suspected to have Dengue and Chikungunya were included. Samples were subjected to IgM antibody capture ELISA kits produced by NIV (Arbovirus Diagnostic NIV, Pune, India). The tests were done according to manufacturer’s instruction. In 2017, Of 1664 samples tested for Dengue, 158(09.49%) were positive and of 1439 samples tested for Chikungunya, 147(10.21%) were positive. In 2018, of 737 samples tested for Dengue, 48 (6.51%) were positive and of 711 samples tested for Chikungunya, 90 (12.6%) were positive. Maximum numbers of Dengue and Chikungunya cases were seen in the age group of 21 to 30 years and 11-20 years respectively. Males were affected higher than females. Maximum seropositives were detected during Monsoon and Pre-monsoon season.The serological tests (ELISA) clearly establish the etiology and also help in initiating appropriate treatment and preventive measures in community.

Keywords

Dengue, Chikungunya, IgM antibody capture ELISA

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