ISSN: 0973-7510

E-ISSN: 2581-690X

Research Article | Open Access
Kawany Gabrieli Zanetti Fazoli1, Isabela Carvalho dos Santos2, Isabel Cristina da Silva Caetano2, Flavio Henrique Carbonera Cervantes3, Filipe Correa Pacheco2, Luiz Antonio Branco2, Ulisses de Padua Pereira4, Lidiane Nunes Barbosa2 and Daniela Dib Goncalves2
1Graduation in Veterinary Medicine, Paranaense University (UNIPAR), Umuarama, Parana, Mascarenhas de Moraes Square, 4282 – Downtown area – 87502-210, Umuarama, Parana, Brazil.
2Post-Graduation Program in Animal Science with Emphasis on Bioactive Products, Scholarship holder CAPES / PROSUP from Paranaense University (UNIPAR), Umuarama, Parana. Mascarenhas de Moraes Square, 4282 – Downtown area – 87502-210, Umuarama, Parana, Brazil.
3Graduation of Agricultural Engineering, Paranaense University (UNIPAR), Umuarama, Parana, Mascarenhas de Moraes Square, 4282 – Downtown area – 87502-210, Umuarama, Parana, Brazil.
4Postgraduate Program in Animal Science – Londrina State University (UEL), Londrina, Parana, Brazil, Celso Garcia Cid Highway, Pr 445, Km 380, University Campus – 86057-970, Londrina, Parana, Brazil.
J Pure Appl Microbiol. 2020;14(2):1149-1156 | Article Number: 6133
https://doi.org/10.22207/JPAM.14.2.10 | © The Author(s). 2020
Received: 04/03/2020 | Accepted: 12/04/2020 | Published: 20/06/2020
Abstract

Lately, bacterial drug resistance has become an important worldwide problem in one health, where bacteria have undergone mutation becoming increasingly resistant. The major problem of bacterial drug resistance is the difficulty with eliminating microorganisms from different wounds and infected patients, and the therapeutic option is most often ineffective as a result of the repeated and inappropriate use of antimicrobials. The objective of this work was to detect and identify enterobacteria, to evaluate their resistance profile and the production of extended-spectrum b-lactamases in draft horse isolates from the municipality of Umuarama, Parana, Brazil. A nasal, oral, and ear cavity swab was collected from 38 horses (used for animal traction) for isolation and bacterial identification, phenotypic antibiotic susceptibility testing, and the phenotypic test for the detection of extended-spectrum b-lactamases-producing strains. In 12 swabs bacterial isolation was possible. Strains of Escherichia coli, Serratia rubidaea, Citrobacterdiversus, Kluyvera species, and Providenciaalcalifaciens were isolated. One hundred percent multidrug resistance was detected, and the antimicrobials that encountered the highest resistance were ertapenem (100%), cefotaxime (100%), cefoxitin (100%), ampicillin (100%), amoxicillin (100%), chloramphenicol (100%), and aztreonam (91.67%) and no extended-spectrum b-lactamases -producing strain was detected. The results of this work reveal the presence of strains of the Enterobacteriaceae family associated with high bacterial resistance in horses used for animal traction in the municipality of Umuarama, State of Parana, Brazil, and these results confirm that these horses can be considered reservoirs of multidrug-resistant microorganisms. This situation can be considered an important problem of one health.

Keywords

Antibiotics, Antimicrobials, Bacteria, Horses, One Health

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