ISSN: 0973-7510

E-ISSN: 2581-690X

Research Article | Open Access

Derick Erl P. Sumalapao1,2 , Frederico Martin D. Alegre2,3, Pauline Bridgette D. Salazar2,3, Nelson R. Villarante4, Isidro C. Sia5,6 and Nina G. Gloriani7

1Department of Epidemiology and Biostatistics, College of Public Health, University of the Philippines Manila, Manila, Philippines.
2Biology Department, College of Science, De La Salle University, Manila, Philippines.
3College of Medicine, De La Salle Medical and Health Sciences Institute, Cavite, Philippines.
4Department of Physical Sciences and Mathematics, College of Arts and Sciences, University of the Philippines Manila, Manila, Philippines.
5Department of Pharmacology and Toxicology, College of Medicine, University of the Philippines Manila, Manila, Philippines.
6Integrative Medicine for Alternative Healthcare Systems Philippines, Inc., Quezon City, Philippines.
7Department of Medical Microbiology, College of Public Health, University of the Philippines Manila, Manila, Philippines.
J Pure Appl Microbiol. 2020;14(2):1143-1148 | Article Number: 6098
https://doi.org/10.22207/JPAM.14.2.09 | © The Author(s). 2020
Received: 19/02/2020 | Accepted: 21/03/2020 | Published: 19/04/2020
Abstract

One of the most prevalent causative organisms in human fungal infections is Candida albicans. The extensive utility of medical devices such as endotracheal tube has resulted in the increased cases of fungal infections. The present study visualized Candida albicans biofilm on polyvinyl chloride endotracheal tube and described the susceptibility profile of the biofilm to antifungal azoles. Biofilm was examined using light and fluorescence microscopy. The susceptibility profile of planktonic and sessile cells with posaconazole, miconazole, voriconazole, and fluconazole was assessed employing the standard disc diffusion method. Planktonic C. albicans was susceptible to the identified antifungal azoles, whereas the sessile cells had varying susceptibility profiles. The biofilms remained susceptible to voriconazole and fluconazole, susceptible-dose dependent to posaconazole, and resistant to miconazole. Findings of the present study can provide relevant information on the resistance mechanism of fungal biofilms with azoles and in the design of new pharmacologic interventions intended as treatment of mycoses.

Keywords

Fluconazole, fungal infections, miconazole, polymer-based material, posaconazole, standard disc diffusion method, voriconazole

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