ISSN: 0973-7510

E-ISSN: 2581-690X

Research Article | Open Access
Muhammad Khalid1, Muhammad Bilal2 , Hira Munir3, Syed Zakir Hussain Shah4, Mohsin Khurshid5, Mohamed El-Shazly6,7 and Hafiz M.N. Iqbal8
1School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai, 200240, China.
2School of Life Science and Food Engineering, Huaiyin Institute of Technology, Huaian 223003, China.
3Department of Biochemistry and Biotechnology, University of Gujrat, Pakistan.
4Department of Zoology, University of Gujrat, Pakistan.
5Department of Microbiology, Government College University Faisalabad, Faisalabad, Pakistan.
6Department of Pharmacognosy, Faculty of Pharmacy, Ain-Shams University, Cairo, Egypt.
7Department of Pharmaceutical Biology, Faculty of Pharmacy and Biotechnology, German University in Cairo, Cairo, Egypt.
8Tecnologico de Monterrey, School of Engineering and Sciences, Campus Monterrey, Ave. Eugenio Garza Sada 2501, Monterrey, N.L., CP 64849, Mexico.
J Pure Appl Microbiol. 2020;14(2):1133-1142 | Article Number: 6300 | © The Author(s). 2020
Received: 08/05/2020 | Accepted: 03/06/2020 | Published: 12/06/2020

The usefulness of medicinal plants has evoked increasing research interests to combat antimicrobial resistance. The current work reports on the in vitro bioactive activities, i.e., antibacterial, anti-biofilm and cytotoxicity evaluation of three medicinal plants. Extracts from different plant parts (leaves, stems, and root barks) were obtained using different solvents, such as methanol, ethanol, and water. The methanolic extracts of bark of Juglans regia and Tamarix aphylla L., whereas the stem of Acacia modesta (Wall.) exhibited the highest antibacterial activity. The maximum zone of inhibition, i.e., 21.8±0.76 mm was observed against H. influenza for a methanolic extract of J. regia. Similarly, the maximum inhibition zones of ethanolic extract of T. aphylla L. against A. baumannii and methanolic extract of A. modesta against S. aureus were recorded. Notably, a higher anti-biofilm potential of the methanolic extract of J. regia was recorded against six pathogenic strains. Finally, the cytotoxicity of plants extracts was evaluated by testing the hemolytic activity against human erythrocytes, that displayed the negligible percent lysis of RBCs. In conclusion, the results of this study provided information related to the possible use of some medicinal plants in the treatment of microbial related ailments.


Medicinal plants, methanolic extract, bacterial pathogens, antibacterial, anti-biofilm, cytotoxicity

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© The Author(s) 2020. Open Access. This article is distributed under the terms of the Creative Commons Attribution 4.0 International License which permits unrestricted use, sharing, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.