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ISSN: 0973-7510

E-ISSN: 2581-690X

Open Access
Shikha Gupta and Sangeeta Pandey
Amity Institute of Organic Agriculture, Amity University Uttar Pradesh, Sector 125, Noida, Uttar Pradesh 201313, India
J Pure Appl Microbiol. 2018;12(2):953-960 | © The Author(s). 2018
Received: 01/04/2018 | Accepted: 19/05/2018 | Published: 30/06/2018

The aim of this present work is to isolate and identify bacterial strains from sweet lime juice sample for bacteriocin production. The cell-free supernatant of these isolates was screened for antimicrobial activity against four test organisms i.e. Bacillus subtilis, Staphylococcus aureus, Escherichia coli and Pseudomonas aeruginosa. Out of  10, three isolates (30%) showed positive inhibition against test organism. The influence of different external parameters on bacteriocin activity as well as on its production was examined. It was found that strain BP03 out of three strains exhibited antimicrobial activity against test pathogens at high temperature (121!), high salt concentration (10% w/v) and over the wide range of pH (3, 6, 7, 9). Further, the morphological and physiological identification was done through Gram staining and biochemical test.


Bacteriocin, Antimicrobial, Juice isolates, biochemical identification, Enterobacter.

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© The Author(s) 2018. Open Access. This article is distributed under the terms of the Creative Commons Attribution 4.0 International License which permits unrestricted use, sharing, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.