ISSN: 0973-7510

E-ISSN: 2581-690X

Open Access
Muayad A. Hussein1 , Hayder A. Muhammid2, Ihab G. AL-Shemmari3 and Yasser J. Jameel4
1Department of Pathology, College of Vet.Med. University of Kerbala. Iraq.
2Department of Microbiology, College of Vet.Med. University of Kerbala. Iraq.
3Department of Vet.Internal and Preventive medicine, College of Vet.Med, University of Kerbala, Iraq.
4Department of Public, College of Vet. Med., University of Kerbala, Iraq.
J Pure Appl Microbiol. 2018;12(2):641-646 | © The Author(s). 2018
Received: 19/02/2018 | Accepted: 02/04/2018 | Published: 30/06/2018

Diagnosis of ovine brucellosis infection in the ram has epididymitis and orchitis   has not been established. The aim of this study, to investigate the diagnostic value of ELISA, Histopathology and PCR for ovine brucellosis in ram in Karbala city. 30 ram’s serum and semen were collected from ram for serological and molecular assay, in addition three testicular tissues were castrated from ram for histopathological diagnosis of Ovine brucellosis. Sensitivity and specificity of ELISA and PCR were 80% ,70%; and 87.5%, 57.14%; respectively, Palpable enlargement of the epididymis and testicular hypoplasia. Sensitivity by ELISA was higher in the ovine brucellosis detection than PCR, but specificity values were lower. Grossly visible lesions in the epididymis associated with Ram Brucellosis with Pocket of inspissate material resulting from extravasation of sperm.


ELISA, Histopathology, PCR Molecular Tests, Brucellosis, Ram

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