ISSN: 0973-7510

E-ISSN: 2581-690X

Open Access
Shahira H. EL-Moslamy
Bioprocess Development Dept., Genetic Engineering and Biotechnology Research Institute (GEBRI), City of Scientific Research and Technology Applications (SRTA-City), New Borg El-Arab city, Alexandria, Egypt.
J Pure Appl Microbiol. 2018;12(2):533-542
https://doi.org/10.22207/JPAM.12.2.12 | © The Author(s). 2018
Received: 23/03/2018 | Accepted: 05/05/2018 | Published: 30/06/2018
Abstract

Nowadays, there are many studies reported the mycosynthesis of nanoparticles by using rhizospheric and epiphytic fungi but there are a few reports using endophytic fungi. So the present work focuses on the mycosynthesis of nano-Ag using endophytic Aspergillus fumigatus strain EAF102 that evaluated for its antifungal efficiency. The fabricated nano-Ag was initially detected by the brown color and UV-VIS spectroscopy that showed the nano-silver peak at 450nm. The structural and morphological properties of the nano-Ag/Ag2O were evaluated by SEM, TEM, XRD, FTIR, EDX and TGA analysis. The mycosynthesized nano- Ag/Ag2O reaction was improved via Taguchi approach and increased 3.9 times larger than the basal reaction condition. The optimized fabrication reaction was set up as following: 1mM of the precursor was added to 25% (v/v) of reductant then this mixture pH was adjusted at 8.0 and the incubation temperature was controlled at 600C under shaking condition (250 rpm). A confirmation test resulted in the maximum nano-Ag/Ag2O was 44.295 ± 5.27 g/l and its S/N ratio was 33.51± 4.20 dB that considered as a very good agreement with the predicted results. The high activities of mycosynthesized nano-Ag/Ag2O (11-15 nm) that used as antiphytopathogens were ordered as follows: Alternaria alternate (3.5±0.36), Fusarium moniliforme (2.2±0.45,) Fusarium graminearum (1.5±0.25), Fusarium semitectum (0.58±0.13), Fusarium oxysporum (0.5±0.23), and Phytophthora arenaria (0.45±0.14). Finally, a simple, fast, and eco-friendly method for mycofabrication of nano-Ag/Ag2O by using endophytic Aspergillus fumigatus strain EAF102 was successfully discovered and improved via this study.

Keywords

Endophytic Aspergillus fumigatus, Mycosynthesis reaction, Nano-Ag/Ag2O, Antiphytopathogens, Taguchi approach

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© The Author(s) 2018. Open Access. This article is distributed under the terms of the Creative Commons Attribution 4.0 International License which permits unrestricted use, sharing, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.