End-stage renal disease (ESRD) is one of serious complications of idiopathic membranous nephropathy (IMN) & it was reported in about one third of patients. IMN is an autoimmune disease in which autoantibodies target antigens at the level of the glomerular basement membrane. Immunological responses may be possibly involved in the pathogenesis of idiopathic membranous nephropatghy (IMN). Cytokines act as a potent immunomodulators. The present study was conducted to  evaluate the role of MIF G173C and TNF-α G308Agenes gene polymorphism in the pathogenesis of IMN. We have investigated single nucleotide polymorphisms of MIF G173C and TNF-α G308A genes in 94 subjects. Forty-six patient had IMN-nephrotic syndrome while 48 subjects were apparently healthy individuals used as a controls ,then the serum level of TNF-á and IL-13 was detected by ELISA technique. The frequencies of  MIF C-173C (13.04 vs 4.00%)  genotypes and C allele (29.35vs 22.00%) were  higher in IMN patients than control group while TNF-α A308A (21.74 vs. 0) genotypes and A allele (38.88 vs 10%) were significantly higher in patient than control groups and associated with higher mean serum concentration of TNF-α (668.33+27.60) versus (45.64+2.38)  and IL-13 (36.70+0.55) versus (2.72+0.22), in IMN patients than apparently healthy subjects. AA genotype with TNF-α-G308A allele polymorphism and CC genotype with MIF 173C  allele are mainly expressed among IMN patients and susceptibility with disease might be prospected.