Jeeranun Untachai1*, Wanwisa Dodgson2,
Akeapot Srifa2 and Jolyon L. A. Dodgson2,3

1Department of Biology, Faculty of Science, Mahasarakham University, Kantarawichai District, Maha Sarakham Province, 44150, Thailand.
2Microbiology and Applied Microbiology Research Unit, Faculty of Science, Mahasarakham University, Kantarawichai District, Maha Sarakham Province, 44150, Thailand.
3Agriculture & Countryside, Myerscough College, Bilsborrow, Preston, Lancashire, PR3 0RY, UK.

Abstract

The present research work was focused on the antibacterial activity of medicinal plants (Aegle marmelosCitrus aurantifoliaPiper sarmentosumSesbania grandifloraCarthamus tinctoriusPiper longumMorus alba, Green tea and Oolong tea). Extracts were examined using water, methanol and ethanol as solvents and tested against six human pathogens (Staphylococcus aureus DMST4212, Bacillus cereus DMST5040, Staphylococcus epidermidisDMST518, Escherichia coli ATCC25922, Methicillin-resistant Staphylococcus aureus (MRSA) DMST20625 and Pseudomonas aeruginosa DMST4739) using the agar well diffusion method. The five day methanol extracts of green tea showed significant activity against MRSA and S. aureus of around 28.3 mm. The five day methanol extracts of A. marmelosexhibited the highest antibacterial activity against S. epidermidis (29.7 mm) and lowest in E. coli (no inhibition zone). The drop plate technique found that three day ethanol and three day methanol extracts of P. longum; water, three day and five day methanol and three day and five day ethanol extracts of green tea and oolong tea; three day and five day methanol and three day and five day ethanol extracts of C. aurantifolia; and three day ethanol extract of S. grandiflora had no growth for all six human pathogens. The results demonstrated that this plant has strong antibacterial potential against all tested bacteria.

Keywords: herb; medicinal plant; antibacterial; extract