ISSN: 0973-7510

E-ISSN: 2581-690X

Open Access
Mona N. Al-Terehi1 , Israa Abd-Alhaleem1, Mohammed R. Abidali2, Zahraa H. Al-Qiam2 and Ali H. Al-Saadi1
1University of Babylon, College of Science, Iraq.
2University of Babylon, College of Nursing, 1Al-Mostaqbal University College, Iraq.
J Pure Appl Microbiol. 2018;12(1):59-63
https://doi.org/10.22207/JPAM.12.1.08 | © The Author(s). 2018
Received: 03/01/2018 | Accepted: 10/03/2018 | Published: 31/03/2018
Abstract

MicroRNAs (miRNAs) are short non-coding RNAs consist of 20-24 nucleotides, it play major roles in all biological pathways in mammals and other multicellular organisms. it effected in numerous cancer-relevant processes like proliferation, cell cycle control, programed cell death, differentiation, migration and metabolism. The present study carried out to estimate  Micr-RNA146a gene polymorphism in breast cancer tissue, about 30 sample of embedded tissue were collected after diagnostic it, also 30 sample of healthy blood were collected for comparison , micro-RNA gene was detected using PCR-SSCP  technique, the results show that there were 4 haplotypes in this gene A,B,C,D . The Variations in these haplotypes appeared  in A and D,  haplotypes A and was appeared in 75%, and 100% in patients and control respectively, D disappeared in control but it was 100% in patients . The present study concluded that this gene can be conducted as risk factor in breast cancer, but it need more investigations to detect SNPs and sequencing of micro-RNA146a gene.

Keywords

Micr-RNA146a,  gene polymorphism, breast cancer, PCR-SSCP.

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