The effect of a commercial probiotics was tested in commercial tiger shrimp, Penaeus monodon (Fabricius) ponds (140+5 days/crop) for two culture periods in Kattumavadi, Tanjavur district, Tamilnadu. Totally 7.99kg/ha probiotics was applied to each experimental pond per crop and remaining ponds are treated as control. Commercial supplementary feed used and feed ration is adjusted to body weight of shrimp. The ponds were stocked with a density of 2300-3000 larval/ha. The water was exchanged in control ponds once in 3 days but not in experimental ponds throughout the culture in two crops. Totally 24 samples were collected for each parameters in each crop. Qualification methods for selected physico-chemical parameters in water and sediments adopted from APHA methods within 48 hrs. There are 11 water quantities parameters in the experimental ponds were analyzed. Among this dissolved oxygen, pH, transparency, salinity, and nitrate shows no significant variation. We found significant variation in BOD, COD, Ammonia, Phosphate, Nitrate and Organic matters. In brief the tested probiotics shows remarkable improvement in physico-chemical status of water and sediment in culture ponds.