In vitro Phosphate Solubilization by Enterobacter spp.isolated from Wheat Rhizosphere

Ishu Bala Thakur and ChayanikaPutatunda*

School of Biotechnology and Biosciences,

Lovely Professional University

Punjab, India

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DOI: http://dx.doi.org/10.22207/JPAM.11.4.43

(Received: 02 September 2017; accepted: 18 Ocotber 2017)

Abstract:

Phosphorus is one of the very important minerals required for the proper plant growth. The availability of phosphorous to plants for uptake and utilization is impaired in alkaline and calcareous soil due to the formation of poorly soluble calcium phosphate minerals. Adding fertilizer phosphorous at normal rates and with conventional methods does not result in optimal yield and crop quality in these soils. The use of phosphate solubilizing bacteria can prove to be effective measure to provide phosphorous to the wheat plants to increase the productivity. In the present investigation, a total of 15 isolates were obtained from wheat rhizosphere soil samples. The isolates were subjected to primary and secondary screening and IKas₄ and IH3₇which showed highest phosphate solubilization during secondary screening  were selected for subsequent studies. The condition for in vitro phosphate solubilization by the selected isolates was optimized. The isolate IH3₇and the isolate IKas₄showed maximum phosphate solubilizationof 0.070 µg and  0.99µg P/ml respectively. The bacterial isolates were gram negative, non-spore forming rods. On the basis of the 16SrDNA sequencing isolates IKas₄ and IH3₇were identified asEnterobacteraerogenesandEnterobactersp. respectively.

Keywords:

Enterobacter, Phosphate solubilizing bacteria, phosphate solubilization, wheat