Saghee Madhu Raju1,2 and Rajkumar Bidlan#2

1Research Scholar, Rayalaseema University, Kurnool, India.
2Dr. Bidlan’s Research Institute, Hyderabad, India.
#Department of Biotechnology, Delhi Technological University, Shahbad Daulatpur,
Delhi- 110042, India.

DOI: http://dx.doi.org/10.22207/JPAM.11.4.36

(Received: 10 October 2017; accepted: 09 December 2017)

Abstract

Organochlorine pesticides (OCPs) such as Lindane and DDT (dichlorodiphenyltrichloroethane) have been extensively used for agricultural purposes primarily for pest management and DDT is stillthe “sought after” for public health care programs to control vector-borne diseases like malaria in developing nations. OCPs, due to recalcitrant nature slowly degrade and pose adverse health effects to the environment and community. Residues of OCPs were detected in soil, water and air leading to potential bioaccumulation in food chainsand were considered persistent organic pollutants. Microorganisms were found to be potential bio-degraders of organochlorine pesticides.

In this study, themicrobial populationfrom aquatic systems, rivers from Yamuna (North India) and Godavari (South India) was isolated and enriched until a Lindane and DDT tolerant population was established. Screening of the population for understanding bioremediation thresholds was done using 5ppm of DDT and Lindane. The populated microbial cells formed the consortium that was used subjected to metagenomic analysis toidentify the organisms till species level. The 16S amplicon sequencing identified 871 species in the consortium and established the biodiversity of the consortium. The defined consortium was able to degrade DDT and Lindane up to 30 ppm simultaneously in varying order of pesticide concentrations.

Keywords:

River Yamuna, River Godavari, Microbial Consortium, Biodegradation, Dichlorodiphenyltrichloroethane (DDT), Hexachlorocyclohexane (HCH), Lindane, Metagenomics, Riverine  Metagenome