*Halemah Mohamed Abulkasim, G. S. Shukla,  H K Bajaj and Harrison Masih.

*Department of Clinical laboratory Sciences-Faculty of Health Science, SHUATS, Allahabad-211007. U.P. (India)

DOI: http://dx.doi.org/10.22207/JPAM.11.4.29

(Received: 01 October 2017; accepted: 30 November 2017)

Abstract:

Throat swabs were collected to isolate S. aureus by using blood agar media. Biochemical tests were performed. Genomic DNA was isolated and PCR was done for ermA, ermB and erm C genes followed by bioinformatics analysis. Isolates were screened on erythromycin disc (erythromycin range 1- 125mg/l). All 47 S. aureus isolates found to be resistance to erythromycin by phenotypic methods contained at least 1 were erythromycin resistance gene. That’s mean all S. aureus isolates which were resistance to erythromycin by disc diffusion method were found as positive by PCR for erm A, B, and C genes.

Keywords: Throat swabs, bacteria,ermA, ermB, ermC.