Open Access

Halemah Mohamed Abulkasim , G.S. Shukla, H.K. Bajaj and Harrison Masih

Department of Clinical laboratory Sciences-Faculty of Health Science, SHUATS, Allahabad-211007. U.P., India.
J Pure Appl Microbiol. 2017;11(4):1891-1897 | © The Author(s). 2017
Received: 01/10/2017 | Accepted: 30/11/2017 | Published: 31/12/2017

Throat swabs were collected to isolate S. aureus by using blood agar media. Biochemical tests were performed. Genomic DNA was isolated and PCR was done for erm A, ermB and erm C genes followed by bioinformatics analysis. Isolates were screened on erythromycin disc (erythromycin range 1- 125mg/l). All 47 S. aureus isolates found to be resistance to erythromycin by phenotypic methods contained at least 1 were erythromycin resistance gene. That’s mean all S. aureus isolates which were resistance to erythromycin by disc diffusion method were found as positive by PCR for erm A, B, and C genes.


Throat swabs, bacteria, erm A, erm B, erm C.

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© The Author(s) 2017. Open Access. This article is distributed under the terms of the Creative Commons Attribution 4.0 International License which permits unrestricted use, sharing, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.