Thamaraikani.I and Kulandhaivel. M

Department of Microbiology,Karpagam Academy of Higher Education, Coimbatore-641 021.

DOI: http://dx.doi.org/10.22207/JPAM.11.4.28

(Received: 10 November 2017; accepted: 21 December 2017)

Abstract:

The aim of the present study is carried out to purify the medicinally potent Hydroxychavicol from methanolic extract of Betel leaf (Piper betle L.) and evaluation of its antimicrobial activity against some of the selected food poison causing pathogens. Hydroxychavicol was successfully purified by silica gel assisted column chromatography. Authenticity of purified Hydroxychavicol was confirmed by Reverse Phase High Performance Liquid Chromatography (RP-HPLC). Minimal Inhibitory Concentration (MIC) and Minimal Bacterial Concentration (MBC) of E.coli and Salmonella were found to be in therange between 20-50 µg. Furthermore, hydroxychavicol was also tested for Time-kill curve and its potential to inhibit the formation of biofilm againstE.coli and Salmonella respectively.In summary, antimicrobial study strongly suggests that thehydroxychavicol possess potent antimicrobial activity against E.coli and Salmonella.

Keywords:

Piper betle, Hydroxychavicol, Antimicrobial suspitability, Food Poison.