Noor S.K. Al-Khafaji* and Azhar  A.L. Al-Thahab

University of Babylon, College of Sciences-Biology Dept. Hilla, Iraq.

DOI: http://dx.doi.org/10.22207/JPAM.11.4.16

(Received: 26 September 2017; accepted: 10 November 2017)

Abstract

Escherichia coli clones, designated as extraintestinal pathogenic E. coli (ExPEC), are responsiblefor extraintestinal infections.  Phylogenetic analysis has shown that E. coli is composed of four main phylogenetic groups (A, B1, B2 and D) and six subgroups (i.e. A0, A1, B22, B23, D1 and D2). Group A and B1 are generally associated with commensals, whereas group B2,D is associated with extra-intestinal pathotypes. In the present study, a total of 53E. coli isolates, isolated from human clinical samples, were used. Phylogenetic grouping was done based on the PCR method using primers targeted at three genetic markers, chuA, yjaAand TspE4.C2. According to PCR-based phylotyping, subgroup B23 contained the majority of the collected isolates ( 40 isolates, 75.47  %), followed by  subgroups A1and B22  (4 isolates for each subgroup, 7.55  %), followed by  subgroups D2( 3 isolates ,5.66 %)  and A0, D1 (1  isolate for each subgroup, 1.88  %). No isolates were found to belong to group B1 . Based on the results, the majority of isolateswere  extra-intestinal pathotypes. Therefore, the role of E. coli in human infections including urinary tract infections, septicemia, vaginitis and meningitis should be considered for further research.

Keywords:

Escherichia coli, Clinical Samples, Iraq.