Malika Talhi-Mekhici1*, Bertrand Cornu2 ,Rahma Talhi1– Mehaya, Djemaia Sahraoui1,Wafaa Dib3, Leila Amel Yazi4,Saidi-Ouahrani Nadjia6, Mourad Kacem5, Corinne Vander Wauven2
1 Laboratoire de Génétique moléculaire, Département de Génétique Moléculaire Appliquée. Université des Sciences et de la Technologie d’Oran Mohamed Boudiaf (USTO-MB), BP1505, Algérie.
2 Institut de Recherches Microbiologiques JM Wiame, Av. E. Gryson 1, B-1070 Bruxelles, Belgique.
3 Laboratoire de Physiologie de la Nutrition et Sécurité Alimentaire, Département de Biologie, Faculté des Sciences, Université d’Oran 1Ahmed Ben Bella, 31000, Algérie.
4 Service de Bactériologie, Etablissement Hospitalier Universitaire d’Oran (EHUO), Algérie
5 Département de Biotechnologie, Faculté des Sciences, Université d’Oran, BP1524, Oran El M’Naouar (31000), Algérie.
6 Laboratoire de biologie du développement et de la différenciation. Université d’Oran 1Ahmed Ben Bella, Algérie.
(Received: 02 October 2017; accepted: 07 November 2017)
Breast-milk is an important source of bacteria for the colonization of the infant‘s gut. The aim of our study was to isolate and identify bacteria from samples of breast-milk of 32 women and from fecal samples of their breast-fed infants. Antimicrobial activity of isolates was also performed. A total of 155 isolates were characterized by phenotypic tests and identified by 16S rDNA sequencing analysis. The isolates belonged to 6 different species of acid lactic bacteria (LAB) and Staphylococcus epidermidis. Enterococcus faecium was the most frequently isolated species (40.8%) and faeces (42.5%). According to the mother’s lifestyle, we noticed that the genus Enterococcus was the most frequently isolated from rural mother’s milk as well as urban mother’s milk. Lactobacillus fermentum (P ≤ 0.05) and Staphylococcus epidermidis (P≤ 0.01) were isolated only from rural mother’s milk. An antimicrobial activity was observed in 30 strains from 148 LAB, the higher level of antagonist waswith E.faecium (35mm). The observed results showed that the isolated strains from rural mother’s milk were different from that urban mother’s milk. Eventual studies can be carried out about lifestyle and nutrition of mothers to explain the effect on the flora found in the milk and feces infants.
human milk, feces infant,bacteria,identification, antimicrobial activity.