Govind Kumar*1, Kavita Arya2, Amit Verma3, Pankaj4, Priyanka Khati4Saurabh Gangola4, Rajesh Kumar5, Anita Sharma4 and Hukum Singh6

1Uttrakand State Council for Science & Technology. India.
2Department of Plant Pathology, G.B.P.U.A.T, Pantnagar, (U.K.), 263145, India.
3Department of Biochemistry, SDAU, Gujarat, India.
4Department of Microbiology, CBSH, G.B.P.U.A.T, Pantnagar (U.K.), India.
5Department of Environmental Microbiology BBAU, Lucknow (UP), India.
6Ecology, Climate change and forest influence Division,
Forest Research Institute, Dehradun, India.


Contamination of soil / water resources by petroleum products poses severe threats to underground water and soil quality. In the present study biosurfactant producing bacterial cultures were used to degrade petrol engine oil under in situ conditions in the plant rhizosphere system. Two bacterial isolates used in this study were recovered from Haldia oil refinery site & petrol station of Pantnagar and identified as Pseudomonas aeruginosa(JX100389) and P. plecoglossicida(JX149549). Application of consortium C2, (Pseudomonas aeruginosa and P. plecoglossicida) degraded 56.14 % petrol engine oil @ 2% in the soil planted with wheat (Triticumaestivum var. 2565) crop after 120 days. GC-MS of biodegraded fuel showed the presence of new product like octanoic acid-2-ethylhexyl ester and 1, 2-benzenedicarboxylic acid.

Keywords: Biophytoremediation; biosurfactant; consortium; bioavailability.